| Gender differences in cholesterol nucleation in native bile: estrogen is a potential contributory factor. | |
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MedLine Citation:
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PMID: 19898891 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The incidence of gallstone disease is two to three times higher in women than in men, and female sex hormones, particularly estrogens, have been implicated as contributory factors. Cholesterol nucleation is the initial step in gallstone pathogenesis and proceeds from cholesterol-rich phospholipid vesicles. The aim of this study was to investigate if there is a difference in cholesterol nucleation rates in male and female bile and whether estrogen influences nucleation rates by interacting with cholesterol-rich regions known as "lipid rafts" that exist within the cholesterol-phospholipid vesicles of the bile. Cholesterol nucleation from native prairie dog bile and the interaction of estrogens with lipid rafts in model bile solutions were investigated using F?rster resonance energy transfer (FRET). Female native bile samples showed a greater reduction in energy transfer than did male native bile, indicating that cholesterol nucleation occurred more readily in female bile than in male bile. Model bile experiments demonstrated that the addition of estrogen has a significant effect, either cholesterol nucleation or raft disruption, but only in samples containing cholesterol-rich rafts. These results suggest that estrogen interacts with cholesterol-rich rafts in vesicles within bile to promote cholesterol nucleation and predispose females to gallstone formation. |
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Authors:
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Angela C Brown; Steven P Wrenn; Nandita Suresh; William C Meyers; Mohammad Z Abedin |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-11-07 |
Journal Detail:
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Title: The Journal of membrane biology Volume: 232 ISSN: 1432-1424 ISO Abbreviation: J. Membr. Biol. Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2010-01-21 Completed Date: 2010-03-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0211301 Medline TA: J Membr Biol Country: United States |
Other Details:
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Languages: eng Pagination: 35-45 Citation Subset: IM |
Affiliation:
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Department of Chemical and Biological Engineering, Drexel University, Philadelphia, PA 19104, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bile / metabolism* Cholelithiasis / etiology* Cholesterol / metabolism* Estrogens / physiology* Female Fluorescence Resonance Energy Transfer Male Membrane Microdomains / physiology Models, Animal Sciuridae Sex Characteristics |
| Chemical | |
Reg. No./Substance:
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0/Estrogens; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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