| Geminin is cleaved by caspase-3 during apoptosis in Xenopus egg extracts. | |
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MedLine Citation:
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PMID: 17651691 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Geminin is an important cell cycle regulator having a dual role in cell proliferation and differentiation. During proliferation, Geminin controls DNA synthesis by interacting with the licensing factor Cdt1 and interferes with the onset of differentiation by inhibiting the activity of transcription factors such as Hox and Six3. During early development Geminin also functions as neural inducer. Thus differential interaction of Geminin with Cdt1 or development-specific transcription factors influence the balance between proliferation and differentiation. Here, we report an additional feature of Geminin showing that it is a novel substrate of caspase-3 during apoptosis in in vitro Xenopus egg extracts. We also show that cleavage of Geminin occurs both in solution and on chromatin with distinct kinetics. In addition we show that cleavage of Geminin by caspase-3 is not relevant to its function as regulator of DNA synthesis, suggesting that its cleavage may be relevant to its role in differentiation. |
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Authors:
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Camille Auziol; Marcel Méchali; Domenico Maiorano |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-07-02 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 361 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2007 Sep |
Date Detail:
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Created Date: 2007-08-07 Completed Date: 2007-09-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 276-80 Citation Subset: IM |
Affiliation:
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Institute of Human Genetics, CNRS-UPR14142, Montpellier, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenine
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analogs & derivatives,
pharmacology Animals Apoptosis* / drug effects Caspase 3 / metabolism* Cell Cycle Proteins / metabolism* Cell Extracts* DNA / biosynthesis DNA Replication / drug effects Mitosis / drug effects Ovum / cytology*, drug effects, enzymology* Xenopus / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Cell Cycle Proteins; 0/Cell Extracts; 0/GMNN protein, Xenopus; 73-24-5/Adenine; 9007-49-2/DNA; 938-55-6/N(6),N(6)-dimethyladenine; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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