| Gel filtration studies on immunoreactive gastrin in serum from Zollinger-Ellison patients. | |
| | |
MedLine Citation:
|
PMID: 4197748 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Sera from 15 patients with the Zollinger-Ellison syndrome were subjected to gel filtration on Sephadex G-50 superfine columns (10 x 2000 mm). The concentration of gastrin in the effluent was determined by a sensitive radioimmunoassay. Immunoreactive gastrin was eluted in four components in 14 sera. (1) Component I, eluted in the same position as proinsulin, constituted 9.7 +/- 1.2 (mean +/- SEM)% of the total immunoreactivity. (2) Component II (;big gastrin') eluted between proinsulin and insulin constituted 57.8 +/- 4.1% (mean +/- SEM) of immunoreactive gastrin. In three sera with the highest concentration of gastrin, component II appeared biphasic. (3) Component III (;little gastrin') was distributed in two peaks; the first one eluted in the same position as the heptadecapeptide gastrin II made up 17.4 +/- 2.7 (mean +/- SEM)% of the total immunoreactivity; the second one eluted in the same position as gastrin I constituted 9.5 +/- 1.3 (mean +/- SEM)%. (4) Component IV (;minigastrin') was eluted immediately before the salt peak and constituted 5.6 +/- 1.4 (mean +/- SEM)%. In one serum only components I and II were present. After incubation with trypsin all immunoreactivity in components I and II was converted to heptadecapeptide-like gastrins.The findings suggest that immunoreactive gastrin in serum from Zollinger-Ellison patients is circulating in at least four components of different molecular size. |
| | |
Authors:
|
J F Rehfeld; F Stadil |
Related Documents
:
|
801698 - Radioimmunoassay of the gastrointestinal hormones. 848248 - Effect of lysine-acetylsalicylate on serum gastrin levels. 4017138 - Effects of meal viscosity on serum gastrin response and gastric emptying rate in duoden... 12643608 - Role of gastric mucosal and gastric juice cytokine concentrations in development of bis... 8535348 - Serum zinc and copper in patients with cerebral vascular disease. 17878248 - Growth hormone suppression after an oral glucose load in children. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Gut Volume: 14 ISSN: 0017-5749 ISO Abbreviation: Gut Publication Date: 1973 May |
Date Detail:
|
Created Date: 1973-09-10 Completed Date: 1973-09-10 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 2985108R Medline TA: Gut Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 369-73 Citation Subset: AIM; IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Chromatography, Ion Exchange Gastrins / blood* Humans Immunodiffusion Insulin Iodine Isotopes Methods Molecular Weight Proinsulin Radioimmunoassay Sodium Isotopes Trypsin Zollinger-Ellison Syndrome / immunology* |
| Chemical | |
Reg. No./Substance:
|
0/Gastrins; 0/Iodine Isotopes; 0/Sodium Isotopes; 11061-68-0/Insulin; 9035-68-1/Proinsulin; EC 3.4.21.4/Trypsin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Serum autoantibodies and immunoglobulins in hepatitis-associated antigen (HAA)-positive and -negativ...
Next Document: Multiplicity of Scrapie virus in infected mouse spleen cells in vivo.