Document Detail

Gel-entrapment of perfluorocarbons: a fluorine-19 NMR spectroscopic method for monitoring oxygen concentration in cell perfusion systems.
MedLine Citation:
PMID:  8429783     Owner:  NLM     Status:  MEDLINE    
Oxygenation is a major determinant of the physiological state of cultured cells. 19F NMR can be used to determine the oxygen concentration available to cells immobilized in a gel matrix by measuring the relaxation rate (1/T1) of perfluorocarbons (PFC) incorporated into the gel matrix. In calcium alginate gel beads without cells the relaxation rate (1/T1) of the trifluoromethyl group of perfluorotripropylamine (FTPA) varies linearly with oxygen concentration, with a slope of 1.26 +/- 0.15 x 10(-3) s-1 microM-1 and an intercept of 0.50 +/- 0.04 s-1. During perfusion with medium equilibrated with 95%/5% O2/CO2, changes in PFC T1s indicate that the average oxygen concentration was reduced from 894 +/- 102 microM in the absence of cells to 476 +/- 65 microM and 475 +/- 50 microM in the presence of 0.7 x 10(8) EMT6/Ro and RIF-1 murine tumor cells per milliliter of gel, respectively. The presence of 0.2 microliters of FTPA/ml of gel had no effect on the energy status of the cells as indicated by 31P NMR spectra. To calculate oxygen gradients within the beads from the average PFC T1 of the sample, a mathematical model was used assuming that oxygen is the limiting nutrient for cell metabolism and that the cellular oxygen consumption rate is independent of oxygen concentration. Data for EMT6/Ro cells were fit using experimentally determined perfusion parameters together with literature values for cell volume and oxygen consumption rate.(ABSTRACT TRUNCATED AT 250 WORDS)
K A McGovern; J S Schoeniger; J P Wehrle; C E Ng; J D Glickson
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine     Volume:  29     ISSN:  0740-3194     ISO Abbreviation:  Magn Reson Med     Publication Date:  1993 Feb 
Date Detail:
Created Date:  1993-03-08     Completed Date:  1993-03-08     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8505245     Medline TA:  Magn Reson Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  196-204     Citation Subset:  IM    
Department of Radiology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287.
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MeSH Terms
Diffusion Chambers, Culture
Glucuronic Acid
Hexuronic Acids
Magnetic Resonance Spectroscopy / diagnostic use,  methods*
Models, Theoretical
Oxygen / analysis
Oxygen Consumption / physiology
Tumor Cells, Cultured / metabolism*
Grant Support
Reg. No./Substance:
0/Alginates; 0/Fluorocarbons; 0/Gels; 0/Hexuronic Acids; 576-37-4/Glucuronic Acid; 7782-41-4/Fluorine; 7782-44-7/Oxygen; 9005-32-7/alginic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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