Document Detail

Gel electrophoretic analysis of histones in lens epithelium, lens fiber, liver, brain, and erythrocytes of late chick embryos.
MedLine Citation:
PMID:  467856     Owner:  NLM     Status:  MEDLINE    
Histones from 19-day-old chick embryo lens epithelium, lens fibers, liver, brain, and erythrocytes were electrophoresed in polyacrylamide gels using buffers containing sodium dodecylsulfate, acetic acid urea, or mixtures of Triton X-100 acetic acid urea. In the last two buffer systems, histone bands were characterized by their apparent molecular weights determined by electrophoresis in the second dimension in sodium dodecylsulfate containing polyacrylamide gels. From the densitograms of the stained gels, the relative proportion of protein in different histone bands was estimated. With the exception of the erythrocyte-specific histone H5, all histones from different tissues examined at any of the gel systems migrated with the same mobilities. In lens epithelium and lens fibers, all histones were present in identical proportions. As compared to liver and brain, the total amount of histone Hl was significantly lower in lens cells and erythrocytes, possibly reflecting differences between the differentiated states. However, no tissue-specific differences were found in the relative distribution of histone Hl I and Hl II among lens epithelium, lens fiber, liver and, brain, but a threefold higher Hl I : Hl II ratio (0.5--0.7) was found in erythrocytes.
C Unger-Ullmann; S P Modak
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Differentiation; research in biological diversity     Volume:  12     ISSN:  0301-4681     ISO Abbreviation:  Differentiation     Publication Date:  1979  
Date Detail:
Created Date:  1979-10-26     Completed Date:  1979-10-26     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0401650     Medline TA:  Differentiation     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  135-44     Citation Subset:  IM    
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MeSH Terms
Brain Chemistry
Chick Embryo / analysis*
Electrophoresis, Polyacrylamide Gel
Epithelium / analysis
Erythrocytes / analysis
Histones / analysis*
Lens, Crystalline / analysis*
Liver / analysis
Time Factors
Reg. No./Substance:

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