Document Detail


Gel-cast glass-ceramic tissue scaffolds of controlled architecture produced via stereolithography of moulds.
MedLine Citation:
PMID:  23013914     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Two glass-ceramic scaffolds with a simple cubic structure of 500 µm square ligaments and square channels of width 400 or 600 µm have been fabricated by gel-casting into moulds produced by stereolithography, followed by mould removal, polymer burnout and sintering. The scaffolds have crushing strengths of 41 ± 14 and 17 ± 5 Mpa, respectively. Using a method of assembling discrete slices of scaffold, we are able to study cell behaviour within a scaffold by disassembly. Both scaffold structures were seeded with primary human osteoblasts and these penetrate, adhere, spread and proliferate on the scaffold structure. The larger channel diameter scaffold shows a greater cell population (despite its smaller surface area) and more pronounced production of ECM components (collagen and mineralization) with increased time in culture. Studies of sectioned scaffolds show that cell density and ECM production decrease with depth and that the difference between the two scaffold architectures is maintained.
Authors:
K Chopra; P M Mummery; B Derby; J E Gough
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-27
Journal Detail:
Title:  Biofabrication     Volume:  4     ISSN:  1758-5090     ISO Abbreviation:  Biofabrication     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101521964     Medline TA:  Biofabrication     Country:  -    
Other Details:
Languages:  ENG     Pagination:  045002     Citation Subset:  -    
Affiliation:
School of Materials, University of Manchester, Oxford Rd, Manchester M13 9PL, UK.
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