Document Detail


Gel-based separation of phosphoproteins in samples stored in urea/thiourea after precipitation by lanthanum chloride.
MedLine Citation:
PMID:  23192385     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The recent introduction of the La(3+) precipitation method for the enrichment of phosphoproteins allows a gel-based analysis of these posttranslationally modified proteins. However, if this method is applied to cell lysates stored in urea-containing lysis buffer for an extended period of time, incomplete phosphoprotein recovery is observed. We ascribe this effect to the presence of urea in the lysis buffer. To overcome this problem various strategies were tested where cell lysates stored at least for one year were utilized. By applying an optimized protocol approx. 250 proteins could be observed following separation by two-dimensional gel electrophoresis.
Authors:
Mario Pink; Christine Stein; Nisha Verma; Albert W Rettenmeier; Simone Schmitz-Spanke
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-28
Journal Detail:
Title:  Electrophoresis     Volume:  -     ISSN:  1522-2683     ISO Abbreviation:  Electrophoresis     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Affiliation:
Institute of Hygiene and Occupational Medicine, University Hospital Essen, Essen, Germany.
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