Document Detail


Gel-based immunotest for simultaneous detection of 2,4,6-trichlorophenol and ochratoxin A in red wine.
MedLine Citation:
PMID:  20579481     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new rapid method which allows simultaneous one step detection of two analytes of different nature (2,4,6,-trichlorophenol (TCP) and ochratoxin A (OTA)) in red wine was developed. It was based on a column test with three separate immunolayers: two test layers and one control layer. Each layer consisted of sepharose gel with immobilized anti-OTA (OTA test layer), anti-TCP (TCP test layer) or anti-HRP (control layer) antibodies. Analytes bind to the antibodies in the corresponding test layer while sample flows through the column. Then a mixture of OTA-HRP and TCP-HRP in appropriate dilutions was used, followed by the application of chromogenic substrate. Colour development of the test layer occurred when the corresponding analyte was absent in the sample. HRP-conjugates bound to anti-HRP antibody in the control layer independently of presence or absence of analytes and a blue colour developed in the control layer. Cut-off values for both analytes were 2 microg L(-1). The described method was applied to the simultaneous detection of TCP and OTA in wine samples. To screen the analytes in red wine samples, clean-up columns were used for sample pre-treatment in combination with the test column. Results were confirmed by chromatographic methods.
Authors:
N V Beloglazova; I Yu Goryacheva; T Yu Rusanova; N A Yurasov; R Galve; M-P Marco; S De Saeger
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-05-24
Journal Detail:
Title:  Analytica chimica acta     Volume:  672     ISSN:  1873-4324     ISO Abbreviation:  Anal. Chim. Acta     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-06-28     Completed Date:  2010-09-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370534     Medline TA:  Anal Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  3-8     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier B.V. All rights reserved.
Affiliation:
Saratov State University, Chemistry Institute, Department of Common and Inorganic Chemistry, Astrakhanskaya 83, 410012 Saratov, Russia.
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MeSH Terms
Descriptor/Qualifier:
Antibodies / immunology
Chlorophenols / analysis*,  immunology
Gels / chemistry
Immunoassay
Ochratoxins / analysis*,  immunology
Wine / analysis*
Chemical
Reg. No./Substance:
0/Antibodies; 0/Chlorophenols; 0/Gels; 0/Ochratoxins; 303-47-9/ochratoxin A; 88-06-2/2,4,6-trichlorophenol

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