Document Detail


A Gel-Encapsulated Bioreactor System for NMR Studies of Protein-Protein Interactions in Living Mammalian Cells.
MedLine Citation:
PMID:  23197368     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Staying alive: The major limitation of in-cell NMR methods is the occurrence of cell death during the NMR measurement. To overcome this problem, a bioreactor was utilized that can perfuse the cells in the NMR tube, thereby maintaining the conditions for greater than 5 h. By using the bioreactor, the binding site of an externally introduced protein for an endogenous molecule in HeLa S3 cells was identified.
Authors:
Satoshi Kubo; Noritaka Nishida; Yuko Udagawa; Osamu Takarada; Shinji Ogino; Ichio Shimada
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-29
Journal Detail:
Title:  Angewandte Chemie (International ed. in English)     Volume:  -     ISSN:  1521-3773     ISO Abbreviation:  Angew. Chem. Int. Ed. Engl.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370543     Medline TA:  Angew Chem Int Ed Engl     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Affiliation:
Division of Physical Chemistry, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033 (Japan).
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