Document Detail

A Gel-Encapsulated Bioreactor System for NMR Studies of Protein-Protein Interactions in Living Mammalian Cells.
MedLine Citation:
PMID:  23197368     Owner:  NLM     Status:  Publisher    
Staying alive: The major limitation of in-cell NMR methods is the occurrence of cell death during the NMR measurement. To overcome this problem, a bioreactor was utilized that can perfuse the cells in the NMR tube, thereby maintaining the conditions for greater than 5 h. By using the bioreactor, the binding site of an externally introduced protein for an endogenous molecule in HeLa S3 cells was identified.
Satoshi Kubo; Noritaka Nishida; Yuko Udagawa; Osamu Takarada; Shinji Ogino; Ichio Shimada
Related Documents :
24677108 - Retinal pigment epithelial cell death by the alternative complement cascade: role of me...
22547408 - Nonpolarized signaling reveals two distinct modes of 3d cell migration.
25129488 - Exploring the link between ceramide and ionizing radiation.
24711698 - Anti-epidermal growth factor receptor sirna carried by chitosan-transacylated lipid nan...
22541538 - Mimicking normal tissue architecture and perturbation in cancer with engineered micro-e...
15845078 - Migration of cells from primary transplants of allo- and xenografted foetal striatal ti...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-29
Journal Detail:
Title:  Angewandte Chemie (International ed. in English)     Volume:  -     ISSN:  1521-3773     ISO Abbreviation:  Angew. Chem. Int. Ed. Engl.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370543     Medline TA:  Angew Chem Int Ed Engl     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Division of Physical Chemistry, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033 (Japan).
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Glycocapture-based proteomics for secretome analysis.
Next Document:  Cost effectiveness of full coverage of the medical management of smoking cessation in France.