| GcvR interacts with GcvA to inhibit activation of the Escherichia coli glycine cleavage operon. | |
| | |
MedLine Citation:
|
PMID: 11495998 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The Escherichia coli glycine cleavage enzyme system, encoded by the gcvTHP operon, catalyses the oxidative cleavage of glycine to CO(2), NH(3) and a one-carbon methylene group. Transcription of the gcv operon is positively regulated by GcvA and negatively regulated by GcvA and GcvR. Using a LexA-based system for analysing protein heterodimerization, it is shown that GcvR interacts directly with GcvA in vivo to repress gcvTHP expression. Several mutations in either gcvA or gcvR that result in a loss of gcv repression also result in a loss of GcvA/GcvR heterodimerization. Finally, it is shown that the C-terminal half of GcvA is involved in its interaction with GcvR, whilst the entire GcvR protein appears to be necessary for heterodimerization. |
| | |
Authors:
|
A C Ghrist; G Heil; G V Stauffer |
Related Documents
:
|
9559548 - Isolation and characterization of kluyveromyces marxianus mutants deficient in malate t... 9674128 - Intracellular distribution of amino acids in an slp1 vacuole-deficient mutant of the ye... 17973458 - Enhanced protein affinity and selectivity of clustered-charge anion-exchange adsorbents. 9030588 - Conversion from archaeal geranylgeranyl diphosphate synthase to farnesyl diphosphate sy... 17775528 - Acid mucopolysaccharide of the crustacean cuticle. 1345768 - An activation of synaptosomal na+,k(+)-atpase by a novel dibenzoxazepine derivative (by... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Microbiology (Reading, England) Volume: 147 ISSN: 1350-0872 ISO Abbreviation: Microbiology (Reading, Engl.) Publication Date: 2001 Aug |
Date Detail:
|
Created Date: 2001-08-09 Completed Date: 2001-12-04 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9430468 Medline TA: Microbiology Country: England |
Other Details:
|
Languages: eng Pagination: 2215-21 Citation Subset: IM |
Affiliation:
|
Department of Microbiology, The University of Iowa, Iowa City, IA 52242, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Oxidoreductases
/
genetics*,
metabolism* Bacterial Proteins / genetics, metabolism* Carrier Proteins / genetics*, metabolism* DNA-Binding Proteins / genetics, metabolism* Escherichia coli / enzymology*, genetics, growth & development Escherichia coli Proteins* Gene Expression Regulation, Bacterial Multienzyme Complexes / genetics*, metabolism* Mutation Operon* Repressor Proteins / genetics, metabolism* Transcription Factors / genetics, metabolism* Transferases / genetics*, metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Bacterial Proteins; 0/Carrier Proteins; 0/DNA-Binding Proteins; 0/Escherichia coli Proteins; 0/GcvA protein, E coli; 0/GcvR protein, E coli; 0/Multienzyme Complexes; 0/Repressor Proteins; 0/Transcription Factors; 0/glycine cleavage system; EC 1.4.-/Amino Acid Oxidoreductases; EC 2.-/Transferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The methylcitric acid pathway in Ralstonia eutropha: new genes identified involved in propionate met...
Next Document: Lipoamide dehydrogenase from Corynebacterium glutamicum: molecular and physiological analysis of the...