Document Detail

GcvR interacts with GcvA to inhibit activation of the Escherichia coli glycine cleavage operon.
MedLine Citation:
PMID:  11495998     Owner:  NLM     Status:  MEDLINE    
The Escherichia coli glycine cleavage enzyme system, encoded by the gcvTHP operon, catalyses the oxidative cleavage of glycine to CO(2), NH(3) and a one-carbon methylene group. Transcription of the gcv operon is positively regulated by GcvA and negatively regulated by GcvA and GcvR. Using a LexA-based system for analysing protein heterodimerization, it is shown that GcvR interacts directly with GcvA in vivo to repress gcvTHP expression. Several mutations in either gcvA or gcvR that result in a loss of gcv repression also result in a loss of GcvA/GcvR heterodimerization. Finally, it is shown that the C-terminal half of GcvA is involved in its interaction with GcvR, whilst the entire GcvR protein appears to be necessary for heterodimerization.
A C Ghrist; G Heil; G V Stauffer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Microbiology (Reading, England)     Volume:  147     ISSN:  1350-0872     ISO Abbreviation:  Microbiology (Reading, Engl.)     Publication Date:  2001 Aug 
Date Detail:
Created Date:  2001-08-09     Completed Date:  2001-12-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9430468     Medline TA:  Microbiology     Country:  England    
Other Details:
Languages:  eng     Pagination:  2215-21     Citation Subset:  IM    
Department of Microbiology, The University of Iowa, Iowa City, IA 52242, USA.
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MeSH Terms
Amino Acid Oxidoreductases / genetics*,  metabolism*
Bacterial Proteins / genetics,  metabolism*
Carrier Proteins / genetics*,  metabolism*
DNA-Binding Proteins / genetics,  metabolism*
Escherichia coli / enzymology*,  genetics,  growth & development
Escherichia coli Proteins*
Gene Expression Regulation, Bacterial
Multienzyme Complexes / genetics*,  metabolism*
Repressor Proteins / genetics,  metabolism*
Transcription Factors / genetics,  metabolism*
Transferases / genetics*,  metabolism*
Reg. No./Substance:
0/Bacterial Proteins; 0/Carrier Proteins; 0/DNA-Binding Proteins; 0/Escherichia coli Proteins; 0/GcvA protein, E coli; 0/GcvR protein, E coli; 0/Multienzyme Complexes; 0/Repressor Proteins; 0/Transcription Factors; 0/glycine cleavage system; EC 1.4.-/Amino Acid Oxidoreductases; EC 2.-/Transferases

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