Document Detail


Gbetagamma is a negative regulator of AP-1 mediated transcription.
MedLine Citation:
PMID:  20403427     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Following stimulation of G protein-coupled receptors (GPCRs) at the cell surface, heterotrimeric G proteins are activated. Both Galpha and Gbetagamma subunits regulate specific effectors to transmit signals received by the receptor. Recent data suggest potential nuclear localization or translocation of the Gbetagamma subunit. Here, we show that co-expression of the Gbetagamma dimer decreased phorbol 12-myristate 13-acetate (PMA)-stimulated AP-1 gene reporter activity in HEK293 cells as well as the AP-1 dependent gonadotropin-releasing hormone-stimulated human follicle-stimulating hormone beta reporter activity in LbetaT2 gonadotrope cells. Further, we identify Fos transcription factors as novel interactors of the Gbeta1 subunit, using protein fragment complementation assays, as well as co-immunoprecipitation in vivo and in vitro. Fos proteins dimerize with Jun proteins to form activator protein-1 (AP-1) transcription factor complexes, which regulate target gene expression. Gbetagamma did not interfere with the dimerization of Fos and Jun or their ability to bind DNA. Rather, Gbetagamma co-localized with the AP-1 complex in the nucleus and recruited histone deacetylases (HDACs) to inhibit AP-1 transcriptional activity. Our data indicate a novel role for Gbetagamma subunits as transcriptional regulators.
Authors:
M?lanie Robitaille; Sarah Gora; Ying Wang; Eug?nie Goupil; Darlaine P?trin; Danny Del Duca; Louis R Villeneuve; Bruce G Allen; St?phane A Laporte; Daniel J Bernard; Terence E H?bert
Related Documents :
16698017 - Nobiletin, a citrus flavonoid, suppresses phorbol ester-induced expression of multiple ...
14714827 - Expression of transcription factors in endometrium during natural cycles.
19225867 - Regulation of c-fos and c-jun gene expression by phospholipase c activity in adult card...
11287607 - Ap-1 repressor protein jdp-2: inhibition of uv-mediated apoptosis through p53 down-regu...
19901527 - How tails define the ending: divergent roles for polyadenylation in rna stability and g...
19001357 - Cardiolipin biosynthesis and remodeling enzymes are altered during development of heart...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-18
Journal Detail:
Title:  Cellular signalling     Volume:  22     ISSN:  1873-3913     ISO Abbreviation:  Cell. Signal.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-05-24     Completed Date:  2010-06-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8904683     Medline TA:  Cell Signal     Country:  England    
Other Details:
Languages:  eng     Pagination:  1254-66     Citation Subset:  IM    
Affiliation:
D?partement de Biochimie, Universit? de Montr?al, Montr?al, Qu?bec, Canada.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cell Nucleus / chemistry
GTP-Binding Protein beta Subunits / analysis,  metabolism*
GTP-Binding Protein gamma Subunits / analysis,  metabolism*
Humans
Mice
Proto-Oncogene Proteins c-fos / metabolism
Rats
Transcription Factor AP-1 / antagonists & inhibitors*,  metabolism
Transcription, Genetic*
Grant Support
ID/Acronym/Agency:
MOP-36279//Canadian Institutes of Health Research; MOP-79354//Canadian Institutes of Health Research; MOP-89991//Canadian Institutes of Health Research
Chemical
Reg. No./Substance:
0/G-protein Beta gamma; 0/GTP-Binding Protein beta Subunits; 0/GTP-Binding Protein gamma Subunits; 0/Proto-Oncogene Proteins c-fos; 0/Transcription Factor AP-1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  NADPH oxidase 4 mediates reactive oxygen species induction of CD146 dimerization in VEGF signal tran...
Next Document:  Sphingosine and FTY720 directly bind pro-survival 14-3-3 proteins to regulate their function.