Document Detail


Gating-ML: XML-based gating descriptions in flow cytometry.
MedLine Citation:
PMID:  18773465     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The lack of software interoperability with respect to gating due to lack of a standardized mechanism for data exchange has traditionally been a bottleneck, preventing reproducibility of flow cytometry (FCM) data analysis and the usage of multiple analytical tools. To facilitate interoperability among FCM data analysis tools, members of the International Society for the Advancement of Cytometry (ISAC) Data Standards Task Force (DSTF) have developed an XML-based mechanism to formally describe gates (Gating-ML). Gating-ML, an open specification for encoding gating, data transformations and compensation, has been adopted by the ISAC DSTF as a Candidate Recommendation. Gating-ML can facilitate exchange of gating descriptions the same way that FCS facilitated for exchange of raw FCM data. Its adoption will open new collaborative opportunities as well as possibilities for advanced analyses and methods development. The ISAC DSTF is satisfied that the standard addresses the requirements for a gating exchange standard.
Authors:
Josef Spidlen; Robert C Leif; Wayne Moore; Mario Roederer; ; Ryan R Brinkman
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Cytometry. Part A : the journal of the International Society for Analytical Cytology     Volume:  73A     ISSN:  1552-4930     ISO Abbreviation:  Cytometry A     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-11-21     Completed Date:  2009-08-11     Revised Date:  2010-09-21    
Medline Journal Info:
Nlm Unique ID:  101235694     Medline TA:  Cytometry A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1151-7     Citation Subset:  IM    
Affiliation:
Terry Fox Laboratory, BC Cancer Agency, Vancouver, British Columbia, Canada.
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MeSH Terms
Descriptor/Qualifier:
Cell Separation / standards*
Flow Cytometry / standards*
Information Storage and Retrieval / standards*
Information Systems / standards*
Software / standards*
Grant Support
ID/Acronym/Agency:
EB005034/EB/NIBIB NIH HHS; R01 EB005034-01/EB/NIBIB NIH HHS; R01 EB005034-02/EB/NIBIB NIH HHS; R01 EB005034-03/EB/NIBIB NIH HHS; R01 EB005034-04/EB/NIBIB NIH HHS
Comments/Corrections

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