Document Detail

Gastrulation in the sea urchin Strongylocentrotus purpuratus is blocked by the fluorescein dye erythrosin B.
MedLine Citation:
PMID:  2168189     Owner:  NLM     Status:  MEDLINE    
Erythrosin B, a food, drug, and cosmetic dye, arrested gastrulation in embryos of the sea urchin Strongylocentrotus purpuratus. A 30 min pulse of 5 microM erythrosin B added at 18 hr postinsemination blocked gastrulation scored at 50 hr postinsemination when control embryos had completed gastrulation. Dye addition at later times had no detectable effects on development through 50 hr postinsemination. The dye may block primary invagination via its known effects on plasma membrane permeability and fluidity.
E J Carroll
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular reproduction and development     Volume:  25     ISSN:  1040-452X     ISO Abbreviation:  Mol. Reprod. Dev.     Publication Date:  1990 Jan 
Date Detail:
Created Date:  1990-10-09     Completed Date:  1990-10-09     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  8903333     Medline TA:  Mol Reprod Dev     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  67-71     Citation Subset:  IM    
Department of Biology, University of California, Riverside 92521.
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MeSH Terms
Cell Membrane Permeability / drug effects
Cells, Cultured
Coloring Agents / administration & dosage,  pharmacology*
Dose-Response Relationship, Drug
Erythrosine / administration & dosage,  pharmacology*
Fluoresceins / administration & dosage,  pharmacology*
Gastrula / drug effects*
Sea Urchins / physiology*
Reg. No./Substance:
0/Coloring Agents; 0/Fluoresceins; 16423-68-0/Erythrosine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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