Document Detail


Gastroprotective effects of oral nucleotide administration.
MedLine Citation:
PMID:  16091553     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND AND AIMS: Nucleotides form the building blocks of DNA and are marketed as dietary supplements, alone or in combination with other ingredients, to promote general health. However, there has been only limited scientific study regarding the true biological activity of orally administered nucleotides. We therefore tested their efficacy in a variety of models of epithelial injury and repair. METHODS: Effects on proliferation ([3H] thymidine incorporation) and restitution (cell migration of wounded monolayers) were analysed using HT29 and IEC6 cells. The ability of a nucleotide mixture to influence gastric injury when administered orally and subcutaneously was analysed using a rat indomethacin (20 mg/kg) restraint model. RESULTS: In both cell lines, cell migration was increased by approximately twofold when added at 1 mg/ml (p<0.01); synergistic responses were seen when a mixture of nucleotides was used. Cell proliferation was stimulated by adenosine monophosphate (AMP) in HT29, but not in IEC6, cells. Gastric injury was reduced by approximately 60% when gavaged at 4-16 mg/ml (p<0.05), concentrations similar to those likely to be found in consumers taking nucleotide supplements. Systemic administration of nucleotides was unhelpful. CONCLUSIONS: Nucleotides possess biological activity when analysed in a variety of models of injury and repair and could provide a novel inexpensive approach for the prevention and treatment of the injurious effects of non steroidal anti-inflammatory drugs and other ulcerative conditions of the bowel. Further studies on their potential benefits (and risks) appear justified.
Authors:
A Belo; T Marchbank; A Fitzgerald; S Ghosh; R J Playford
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-08-09
Journal Detail:
Title:  Gut     Volume:  55     ISSN:  0017-5749     ISO Abbreviation:  Gut     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-01-12     Completed Date:  2006-02-13     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985108R     Medline TA:  Gut     Country:  England    
Other Details:
Languages:  eng     Pagination:  165-71     Citation Subset:  AIM; IM    
Affiliation:
Department of Gastroenterology, Imperial College Faculty of Medicine, Hammersmith Hospital, Ducane Rd, London W12 0NN, UK.
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MeSH Terms
Descriptor/Qualifier:
Administration, Oral
Animals
Anti-Inflammatory Agents, Non-Steroidal / toxicity
Cell Movement / drug effects
Cell Proliferation / drug effects
Dietary Supplements*
Dose-Response Relationship, Drug
Drug Combinations
Humans
Indomethacin / toxicity
Injections, Subcutaneous
Male
Nucleotides / pharmacology*,  therapeutic use
Rats
Rats, Sprague-Dawley
Stomach Diseases / chemically induced,  prevention & control*
Tumor Cells, Cultured
Wound Healing / drug effects
Grant Support
ID/Acronym/Agency:
//Wellcome Trust
Chemical
Reg. No./Substance:
0/Anti-Inflammatory Agents, Non-Steroidal; 0/Drug Combinations; 0/Nucleotides; 53-86-1/Indomethacin
Comments/Corrections

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