| Gastrin-releasing peptide is a transmitter mediating porcine gallbladder contraction. | |
| | |
MedLine Citation:
|
PMID: 1708207 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We studied the role of gastrin-releasing peptide (GRP) for porcine gallbladder motility. Immunohistochemistry visualized nerve fibers containing GRP-like immunoreactivity in muscularis. GRP concentration dependently stimulated contractions of muscularis strips (ED50, 2.9 nM). Neuromedin B was less potent (ED50, 0.1 microM), suggesting existence of GRP-preferring receptors. GRP-induced contractions were unaffected by muscarinic antagonism (1 microM atropine), axonal blockade (1 microM tetrodotoxin), cholecystokinin (CCK) receptor antagonism (10 microM MK-329), or substance P desensitization (1 microM), supporting the existence of myogenic GRP receptors. The bombesin (BN) analogue D-Phe6-BN-(6-13)propylamide (PA) stimulated contractions (ED50, 3.3 nM) with low efficacy (29% of that of GRP). D-Phe6-BN-(6-13)PA (1 microM) shifted GRP concentration-response curves one log to the right. D-Phe6-BN-(6-13)PA interacted specifically with GRP receptors; while abolishing responses to GRP (1 nM), responses to substance P (0.1 microM) and CCK-8 (1 nM) were unchanged. Electrical stimulation (10 Hz, 0.5 ms, 10 V) caused a rapid onset-slow offset, tetrodotoxin-sensitive excitation. Atropine reduced the amplitude to 58% and caused a delayed, slow onset-slow decline response. D-Phe6-BN-(6-13)PA reduced the amplitude to 59% and caused a very rapid onset-rapid decline response. Atropine plus D-Phe6-BN-(6-13)PA abolished responses to nerve stimulation. Nerve stimulation caused significant release of GRP-like immunoreactivity. Thus two neural inputs were defined: a cholinergic rapid onset-rapid offset excitation and a delayed, slow onset-slow offset excitation caused by release and subsequent binding of GRP to GRP-preferring receptors. |
| | |
Authors:
|
B Schjoldager; S S Poulsen; P Schmidt; D H Coy; J J Holst |
Related Documents
:
|
2417207 - Substance k and substance p differentially modulate mesolimbic and mesocortical systems. 2462417 - Effect of the n-terminal tetrapeptide of substance p sp (1-4) and tuftsin on the pre- a... 8827457 - Developmental changes of neutral glycosphingolipids as receptors for pulmonary surfacta... 11967897 - Cellular basis for the effects of substance p in the periaqueductal gray and dorsal rap... 10336567 - Adenosine kinase inhibitors as a novel approach to anticonvulsant therapy. 17289027 - Distinct fos expression in the brain following freezing behavior elicited by stimulatio... |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The American journal of physiology Volume: 260 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1991 Apr |
Date Detail:
|
Created Date: 1991-05-17 Completed Date: 1991-05-17 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: G577-85 Citation Subset: IM |
Affiliation:
|
Department of Medical Physiology C, Panum Institute, University of Copenhagen, Denmark. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Atropine / pharmacology Carbachol / pharmacology Electric Stimulation Gallbladder / drug effects, innervation, physiology* Gastrin-Releasing Peptide Gastrointestinal Hormones / pharmacology* Kinetics Muscle Contraction / drug effects* Muscle, Smooth / drug effects, innervation, physiology* Nerve Fibers / physiology Neurokinin B / analogs & derivatives, pharmacology Peptides / analysis, pharmacology*, physiology Sincalide / pharmacology Substance P / analysis, pharmacology, physiology Swine Tetrodotoxin / pharmacology |
| Chemical | |
Reg. No./Substance:
|
0/Gastrointestinal Hormones; 0/Peptides; 25126-32-3/Sincalide; 33507-63-0/Substance P; 4368-28-9/Tetrodotoxin; 51-55-8/Atropine; 51-83-2/Carbachol; 80043-53-4/Gastrin-Releasing Peptide; 86933-75-7/Neurokinin B; 87096-84-2/neuromedin B |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Sarcosine kinetics in pigs by infusion of [1-14C]sarcosine: use for refining estimates of glycine an...
Next Document: Slow-wave activity in colon: role of network of submucosal interstitial cells of Cajal.