Document Detail

Gastric motility in ghrelin receptor knockout mice.
MedLine Citation:
PMID:  23128468     Owner:  NLM     Status:  Publisher    
The aim of this study was to investigate the effects and possible mechanisms of ghrelin receptor (GHS-R) deficiency on gastric motility in GHS-R deficient (Ghsr-/-) mice. Ghsr-/- and control (Ghsr+/+) mice were genotyped by PCR. The percentage of gastric emptying (GE%) was calculated following the intraperitoneal adminis-tration of ghrelin. In vitro, the contractile response of smooth muscle strips to ghrelin and electrical field stimulation (EFS) and the intraluminal pressure change of isolated stomach to carbachol were observed in an organ bath. The staining of nerve cells in the gastric muscle layer was performed by immunofluorescence. Delayed gastric emptying was observed in the Ghsr-/- mice; ghrelin enhanced the GE% in the Ghsr+/+ mice but had no effect on the GE% in the Ghsr-/- mice. In vitro, the response of the strips to ghrelin and EFS and the intraluminal pressure change to cabarchol was reduced in the Ghsr-/- mice. GHS-Rs were predominantly expressed on nerve cells in gastric muscle layers. The number of nerve cells was observed to be decreased in the Ghsr-/- mice. The delayed gastric emptying may relate to the loss of GHS-Rs and the reduction in the number of nerve cells in the gastric muscle layers of the GHS-R-deficient mice.
Cheng-Guang Yang; Wei-Gang Wang; Jun Yan; Jian Fei; Zhi-Gang Wang; Qi Zheng
Related Documents :
16733698 - Inhibitors of alpha-synuclein oligomerization and toxicity: a future therapeutic strate...
10541868 - Alpha-synuclein immunoreactive lewy bodies and lewy neurites in parkinson's disease are...
11080208 - Sensitization of neuronal cells to oxidative stress with mutated human alpha-synuclein.
11934688 - Alpha-melanocyte-simulating hormone and interleukin-10 do not protect the kidney agains...
11449378 - Ifn-alpha and il-18 synergistically enhance ifn-gamma production in human nk cells: dif...
1664838 - Staphylococcus aureus mastitis: pathogenesis and treatment with bovine interleukin-1 be...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-29
Journal Detail:
Title:  Molecular medicine reports     Volume:  -     ISSN:  1791-3004     ISO Abbreviation:  Mol Med Report     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-11-6     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101475259     Medline TA:  Mol Med Report     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of General Surgery, The Affiliated Sixth Hospital of Medical School, Shanghai Jiaotong University, Shanghai 200233, P.R. China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  N-acetylcysteine potentiates doxorubicin-induced ATM and p53 activation in ovarian cancer cells.
Next Document:  Response to vitamin D3 supplementation in obese and non-obese Caucasian adolescents.