Document Detail

Gastric emptying and Ca(2+) and K(+) channels of circular smooth muscle cells in diet-induced obese prone and resistant rats.
MedLine Citation:
PMID:  23404843     Owner:  NLM     Status:  Publisher    
Accelerated gastric emptying which precipitates hunger and frequent eating could be a potential factor in the development of obesity. The aim of this study was to study gastric emptying in diet-induced obese-Prone (DIO-P) and DIO-Resistant (DIO-R) rats and explore possible differences in electrical properties of calcium (Ca(2+) ) and potassium (K(+) ) channels of antral circular smooth muscle cells (SMCs). Whole-cell patch clamp technique was used to measure Ca(2+) and K(+) currents in single SMCs. Gastric emptying was evaluated 90 min after the ingestion of a solid meal. Solid gastric emptying in the DIO-P rats was significantly faster compared to that in the DIO-R rats. The peak amplitude of L-type Ca(2+) current (I(Ba,L) ) at 10 mV in DIO-P rats was greater than that in DIO-R rats without alternation of the current-voltage curve and voltage dependent activation and inactivation. The half maximal inactivation voltage of transient outward K(+) current (I(Kto) ) was more depolarized (∼4 mV) in DIO-P rats compared with that in DIO-R rats. No difference was found in the current density or recovery kinetics of I(Kto) between two groups. The current density of delayed rectifier K(+) current (I(Kdr) ), which was sensitive to tetraethylammonium chloride but not 4-aminopyridine was lower in DIO-P rats than that in DIO-R rats. Our data suggested that the accelerated gastric emptying in DIO-P rats might be attributed to a higher density of I(Ba,L) , depolarizing shift of inactivation curve of I(Kto) and lower density of I(Kdr) observed in the antral SMCs of DIO-P rats.
Shiying Li; Roland Maude-Griffin; Andrew J Pullan; Jiande D Z Chen
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-28
Journal Detail:
Title:  Obesity (Silver Spring, Md.)     Volume:  -     ISSN:  1930-739X     ISO Abbreviation:  Obesity (Silver Spring)     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2013-2-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101264860     Medline TA:  Obesity (Silver Spring)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 The Obesity Society.
Veterans Research and Education Foundation, VA Medical Center, Oklahoma City, OK.
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