Document Detail

Gastric carcinoma: monoclonal epithelial malignant cells expressing Epstein-Barr virus latent infection protein.
MedLine Citation:
PMID:  8090780     Owner:  NLM     Status:  MEDLINE    
In 1000 primary gastric carcinomas, 70 (7.0%) contained Epstein-Barr virus (EBV) genomic sequences detected by PCR and Southern blots. The positive tumors comprised 8 of 9 (89%) undifferentiated lymphoepithelioma-like carcinomas, 27 of 476 (5.7%) poorly differentiated adenocarcinomas, and 35 of 515 (6.8%) moderately to well-differentiated adenocarcinomas. In situ EBV-encoded small RNA 1 hybridization and hematoxylin/eosin staining in adjacent sections showed that the EBV was present in every carcinoma cell but was not significantly present in lymphoid stroma and in normal mucosa. Two-color immunofluorescence and hematoxylin/eosin staining in parallel sections revealed that every keratin-positive epithelial malignant cell expressed EBV-determined nuclear antigen 1 (EBNA1) but did not significantly express CD45+ infiltrating leukocytes. A single fused terminal fragment was detected in each of the EBNA1-expressing tumors, thereby suggesting that the EBV-carrying gastric carcinomas represent clonal proliferation of cells infected with EBV. The carcinoma cells had exclusively EBNA1 but not EBNA2, -3A, -3B, and -3C; leader protein; and latent membrane protein 1 because of methylation. The patients with EBV-carrying gastric carcinoma had elevated serum EBV-specific antibodies. The EBV-specific cellular immunity was not significantly reduced; however, the cytotoxic T-cell target antigens were not expressed. These findings strongly suggest a causal relation between a significant proportion of gastric carcinoma and EBV, and the virus-carrying carcinoma cells may evade immune surveillance.
S Imai; S Koizumi; M Sugiura; M Tokunaga; Y Uemura; N Yamamoto; S Tanaka; E Sato; T Osato
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  91     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1994 Sep 
Date Detail:
Created Date:  1994-10-14     Completed Date:  1994-10-14     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  9131-5     Citation Subset:  IM    
Department of Virology, Hokkaido University School of Medicine, Sapporo, Japan.
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MeSH Terms
Antibodies, Viral / immunology
Antigens, Viral / genetics,  metabolism*
Carcinoma / immunology,  microbiology*,  pathology
Clone Cells
DNA, Neoplasm / genetics
DNA-Binding Proteins / genetics,  metabolism*
Epstein-Barr Virus Nuclear Antigens
Genes, Viral
Herpesvirus 4, Human / immunology,  pathogenicity*
Immunity, Cellular
Stomach Neoplasms / immunology,  microbiology*,  pathology
Tumor Virus Infections / immunology,  microbiology*
Viral Structural Proteins / genetics
Reg. No./Substance:
0/Antibodies, Viral; 0/Antigens, Viral; 0/DNA, Neoplasm; 0/DNA-Binding Proteins; 0/Epstein-Barr Virus Nuclear Antigens; 0/Viral Structural Proteins

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