Document Detail


Gastric antibacterial efficiency is different for pepsin A and C.
MedLine Citation:
PMID:  16333616     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The gastric lumen represents a bactericidal barrier, whose major components are an acidic pH and a family of isoenzymes of the gastric aspartate protease, pepsin. To evaluate whether specific pepsins are specialized in antibacterial protection, we tested their effects on the gastric pathogen Helicobacter pylori. In a recent study we found pepsin to affect the motility of the bacteria, one of its most important virulence factors. We were able to show that the antibacterial effect of pepsin occurs in two phases: rapid loss of motility and subsequent destruction. In the present study we used the rapid pepsin-induced bacterial immobilization as a marker of antibacterial efficiency. The proteolytic activity of different pepsins was normalized to values between 2 and 200 U/ml in the hemoglobin degradation test of Anson, performed at pH 2 and 5. We found that pepsin C completely inactivates H. pylori at proteolytic activities of 2 (pH 5) and 20 (pH 2) U/ml. In contrast, the activities of pepsin A and chymosin required to affect Helicobacter motility were ten times higher.
Authors:
Sören Schreiber; Roland Bücker; Claudia Groll; Marina Azevedo-Vethacke; Peter Scheid; Sören Gatermann; Christine Josenhans; Sebastian Suerbaum
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-12-07
Journal Detail:
Title:  Archives of microbiology     Volume:  184     ISSN:  0302-8933     ISO Abbreviation:  Arch. Microbiol.     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2005-12-19     Completed Date:  2006-03-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0410427     Medline TA:  Arch Microbiol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  335-40     Citation Subset:  IM    
Affiliation:
Institut für Physiologie, MA 2/149, Ruhr-Universität Bochum, Universitätsstrasse 150, 44801, Bochum, Germany. soeren.schreiber@rub.de
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MeSH Terms
Descriptor/Qualifier:
Animals
Anti-Bacterial Agents / pharmacology*
Cattle
Chymosin / pharmacology
Gastric Mucosa / microbiology*
Helicobacter pylori / drug effects*,  growth & development,  physiology*
Humans
Hydrogen-Ion Concentration
Microbial Sensitivity Tests
Pepsin A / pharmacology*
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; EC 3.4.23.1/Pepsin A; EC 3.4.23.3/gastricsin; EC 3.4.23.4/Chymosin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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