Document Detail

Gas chromatography/isotope ratio mass spectrometry of recalcitrant target compounds: performance of different combustion reactors and strategies for standardization.
MedLine Citation:
PMID:  22467455     Owner:  NLM     Status:  In-Data-Review    
RATIONALE: Compound-specific isotope analysis (CSIA) relies on continuous flow combustion of organic substances to CO(2) and N(2) in a miniature reactor to measure (13)  C/(12)  C and (15)  N/(14)  N stable isotope ratios. Accurate analysis is well established for many volatile hydrocarbons. In contrast, compounds which contain hetero and halogen atoms are less volatile and may be more recalcitrant to combustion.
METHODS: This study tested carbon and nitrogen isotope analysis of atrazine, desethylatrazine (DEA), dichlobenil and 2,6-dichlorobenzamide (BAM) by gas chromatography/isotope ratio mass spectrometry (GC/IRMS) with multiple reactor tubes of two different kinds (conventional CuO/NiO/Pt and a NiO tube/CuO-NiO reactor prototype).
RESULTS: The advantages of the NiO tube/CuO-NiO reactor were the absence of an additional reduction reactor, the possibility of routine reoxidation in nitrogen isotope analysis, and reliable atrazine and DEA measurements over several hundred injections. In contrast, BAM analysis showed good accuracy for carbon, but notable variations in the trueness of nitrogen isotope ratios. Accurate carbon and nitrogen analysis was nevertheless possible by bracketing samples with external compound-specific standards and subsequent offset correction.
CONCLUSIONS: We conclude that instrument data should never be taken at its 'face value', but must consistently be validated with compound-specific standards of the respective analytes. Copyright © 2012 John Wiley & Sons, Ltd.
Sandra Reinnicke; Dieter Juchelka; Sibylle Steinbeiss; Armin Meyer; Andreas Hilkert; Martin Elsner
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Rapid communications in mass spectrometry : RCM     Volume:  26     ISSN:  1097-0231     ISO Abbreviation:  Rapid Commun. Mass Spectrom.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-04-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8802365     Medline TA:  Rapid Commun Mass Spectrom     Country:  England    
Other Details:
Languages:  eng     Pagination:  1053-60     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 John Wiley & Sons, Ltd.
Institute of Groundwater Ecology, Helmholtz Zentrum Muenchen, Ingolstaedter Landstr. 1, 85764, Neuherberg, Germany.
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