Document Detail


Gas-phase sequencing after electroblotting on polyvinylidene difluoride membranes assigns correct molecular weights to myoglobin molecular weight markers.
MedLine Citation:
PMID:  2619034     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Commercially available polypeptide marker kits containing peptides generated by cyanogen bromide cleavage of either horse heart myoglobin or sperm whale myoglobin have been investigated by sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE), followed by electroblotting on polyvinylidene difluoride membranes, and gas-phase sequencing. It could be shown that the molecular weights assigned to the SDS-PAGE bands by the companies are incorrect. Arranged in descending order, the marker kits are composed of the following polypeptide fragments from myoglobin: positions 1-153, 1-131, 56-153, 56-131, 1-55, and 132-153. A polypeptide comprising residues 1-14 was not found. According to these results the log Mr versus Rf plot used for calibration must be revised. For the separation of low molecular weight polypeptides and peptides a new gel system based on the theory of multiphasic zone electrophoresis combined with a modified Coomassie staining procedure is reported.
Authors:
H D Kratzin; J Wiltfang; M Karas; V Neuhoff; N Hilschmann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical biochemistry     Volume:  183     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1989 Nov 
Date Detail:
Created Date:  1990-03-08     Completed Date:  1990-03-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1-8     Citation Subset:  IM    
Affiliation:
Max-Planck-Institut für experimentelle Medizin, Abteilung für Immunchemie, Goettingen, West Germany.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence*
Animals
Electrophoresis, Polyacrylamide Gel / methods
Horses
Membranes, Artificial
Molecular Sequence Data
Molecular Weight
Myoglobin / analysis*
Polyvinyls
Chemical
Reg. No./Substance:
0/Membranes, Artificial; 0/Myoglobin; 0/Polyvinyls; 24937-79-9/polyvinylidene fluoride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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