Document Detail

Gas chromatography/time-of-flight mass spectrometry-based metabonomics of hepatocarcinoma in rats with lung metastasis: elucidation of the metabolic characteristics of hepatocarcinoma at formation and metastasis.
MedLine Citation:
PMID:  20814984     Owner:  NLM     Status:  MEDLINE    
Hepatocarcinoma (HCC) has a very high mortality rate and the high recurrence and metastasis rates contribute to the poor prognosis of HCC patients. To understand HCC formation and metastasis, we assessed the metabonomics of rat HCC and HCC with lung metastasis (HLM). The HLM rat model was established by exposure to diethylnitrosamine (DEN). Levels of serum and urine metabolites were quantified with gas chromatography/time-of-flight mass spectrometry (GC/TOFMS), and data were analyzed with partial least-squares discrimination analysis (PLS-DA). Serum and urine levels of some metabolites differed significantly between the control, HCC, and HLM groups. The products and intermediates from glycolysis and glutamate metabolism were elevated, while the tricarboxylic acid (TCA) cycle was inhibited, in both HCC and HLM. HLM samples revealed enhanced metabolism of nucleic acids, amino acids and glucuronic acid. PLS-DA indicated that principal component weighting was greatest for serum serine, phenylalanine, lactic acid, tyrosine and glucuronic acid, and urine glycine, serine, 5-oxyproline, malate, hippuric acid and uric acid. These data provide novel information that will improve understanding of the pathophysiological processes involved in HCC and HLM, and revealed potential metabolic markers for HCC invasion and metastasis.
Zong-Fang Li; Juan Wang; Chen Huang; Shu Zhang; Jun Yang; An Jiang; Rui Zhou; Dun Pan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Rapid communications in mass spectrometry : RCM     Volume:  24     ISSN:  1097-0231     ISO Abbreviation:  Rapid Commun. Mass Spectrom.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-03     Completed Date:  2011-01-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8802365     Medline TA:  Rapid Commun Mass Spectrom     Country:  England    
Other Details:
Languages:  eng     Pagination:  2765-75     Citation Subset:  IM    
Copyright Information:
2010 John Wiley & Sons, Ltd.
Department of General Surgery, The Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an 710004, China.
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MeSH Terms
Carcinoma / chemically induced,  metabolism*,  pathology,  secondary
Computational Biology
Gas Chromatography-Mass Spectrometry / methods*
Least-Squares Analysis
Liver Neoplasms, Experimental / chemically induced,  metabolism*,  pathology
Lung Neoplasms / metabolism,  secondary*
Metabolomics / methods*
Multivariate Analysis
Pattern Recognition, Automated
Rats, Sprague-Dawley
Tumor Markers, Biological / blood,  chemistry,  urine
Reg. No./Substance:
0/Tumor Markers, Biological; 55-18-5/Diethylnitrosamine

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