| Gas chromatography/time-of-flight mass spectrometry-based metabonomics of hepatocarcinoma in rats with lung metastasis: elucidation of the metabolic characteristics of hepatocarcinoma at formation and metastasis. | |
| | |
MedLine Citation:
|
PMID: 20814984 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Hepatocarcinoma (HCC) has a very high mortality rate and the high recurrence and metastasis rates contribute to the poor prognosis of HCC patients. To understand HCC formation and metastasis, we assessed the metabonomics of rat HCC and HCC with lung metastasis (HLM). The HLM rat model was established by exposure to diethylnitrosamine (DEN). Levels of serum and urine metabolites were quantified with gas chromatography/time-of-flight mass spectrometry (GC/TOFMS), and data were analyzed with partial least-squares discrimination analysis (PLS-DA). Serum and urine levels of some metabolites differed significantly between the control, HCC, and HLM groups. The products and intermediates from glycolysis and glutamate metabolism were elevated, while the tricarboxylic acid (TCA) cycle was inhibited, in both HCC and HLM. HLM samples revealed enhanced metabolism of nucleic acids, amino acids and glucuronic acid. PLS-DA indicated that principal component weighting was greatest for serum serine, phenylalanine, lactic acid, tyrosine and glucuronic acid, and urine glycine, serine, 5-oxyproline, malate, hippuric acid and uric acid. These data provide novel information that will improve understanding of the pathophysiological processes involved in HCC and HLM, and revealed potential metabolic markers for HCC invasion and metastasis. |
| | |
Authors:
|
Zong-Fang Li; Juan Wang; Chen Huang; Shu Zhang; Jun Yang; An Jiang; Rui Zhou; Dun Pan |
Related Documents
:
|
11339284 - Analysis of the metabolites of the sodium salt of 6-hydroxy-5-(phenylazo)-2-naphthalene... 10574204 - Determination of urinary orotic acid and uracil by capillary zone electrophoresis. 12373394 - First- and second-order multivariate calibration applied to biological samples: determi... 11282094 - Urine 4-heptanone: a beta-oxidation product of 2-ethylhexanoic acid from plasticisers. 20668164 - Intracellular phospholipase a1 and acyltransferase, which are involved in caenorhabditi... 6141914 - Effects of phenobarbital, 3-methylcholanthrene, and retinoid pretreatment on dispositio... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Rapid communications in mass spectrometry : RCM Volume: 24 ISSN: 1097-0231 ISO Abbreviation: Rapid Commun. Mass Spectrom. Publication Date: 2010 Sep |
Date Detail:
|
Created Date: 2010-09-03 Completed Date: 2011-01-04 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8802365 Medline TA: Rapid Commun Mass Spectrom Country: England |
Other Details:
|
Languages: eng Pagination: 2765-75 Citation Subset: IM |
Copyright Information:
|
2010 John Wiley & Sons, Ltd. |
Affiliation:
|
Department of General Surgery, The Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an 710004, China. lzf2568@gmail.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Carcinoma / chemically induced, metabolism*, pathology, secondary Computational Biology Diethylnitrosamine Gas Chromatography-Mass Spectrometry / methods* Histocytochemistry Least-Squares Analysis Liver Neoplasms, Experimental / chemically induced, metabolism*, pathology Lung Neoplasms / metabolism, secondary* Male Metabolomics / methods* Multivariate Analysis Pattern Recognition, Automated Rats Rats, Sprague-Dawley Tumor Markers, Biological / blood, chemistry, urine |
| Chemical | |
Reg. No./Substance:
|
0/Tumor Markers, Biological; 55-18-5/Diethylnitrosamine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Online coupling of thin layer chromatography with matrix-assisted laser desorption/ionization time-o...
Next Document: Double disruption of ?(2A) - and ?(2C) -adrenoceptors results in sympathetic hyperactivity and high-...