Document Detail


Gas chromatography-tandem mass spectrometry analysis of gabapentin in serum.
MedLine Citation:
PMID:  15470697     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new highly sensitive analytical method for determining gabapentin [1-(aminomethyl) cyclohexaneacetic acid; Neurontin] in serum using gas chromatography/tandem mass spectrometry (GC-MS/MS) was developed. GC-MS/MS was applied to determine the levels of gabapentin in serum samples of mice at 1 and 6 h after oral or intraperitoneal treatment (300 mg/kg). At 1 h, the concentrations of the drug were 4.02 +/- 0.42 and 4.32 +/- 0.28 microg/mL in mice treated orally and intraperitoneally, respectively. At 6 h, drug levels decreased by about 66% in both groups. The method, coupling two stages of mass analysis, could be very useful in identifying the drug in complex mixtures such as blood and urine. Moreover, it is easy and rapid to perform, and sensitive enough to allow the presence of the drug to be determined at very low detection limits. It is a very reliable method for both clinical and experimental monitoring of gabapentin.
Authors:
Cristiana Gambelunghe; Giuseppina Mariucci; Michela Tantucci; Maria Vittoria Ambrosini
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biomedical chromatography : BMC     Volume:  19     ISSN:  0269-3879     ISO Abbreviation:  Biomed. Chromatogr.     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2005-02-21     Completed Date:  2005-07-12     Revised Date:  2013-06-11    
Medline Journal Info:
Nlm Unique ID:  8610241     Medline TA:  Biomed Chromatogr     Country:  England    
Other Details:
Languages:  eng     Pagination:  63-7     Citation Subset:  IM    
Affiliation:
Department of Clinical and Experimental Medicine-Laboratorio delle Attività Motorie e Sportive, University of Perugia, Perugia, Italy.
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MeSH Terms
Descriptor/Qualifier:
Amines / blood*
Animals
Cyclohexanecarboxylic Acids / blood*
Gas Chromatography-Mass Spectrometry / methods*
Male
Mice
Sensitivity and Specificity
gamma-Aminobutyric Acid / blood*
Chemical
Reg. No./Substance:
0/Amines; 0/Cyclohexanecarboxylic Acids; 56-12-2/gamma-Aminobutyric Acid; 6CW7F3G59X/gabapentin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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