Document Detail


Gas chromatography-tandem mass spectrometry determination of 17alpha-ethinylestradiol residue in the hair of cattle. Application to treated animals.
MedLine Citation:
PMID:  11804509     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A gas chromatography-tandem mass spectrometry (GC-MS(2)) method for the detection and quantification of 17alpha-ethinylestradiol in the hair of cattle has been developed, and uses an ion trap analyzer. After the digestion of 500 mg of hair by alkaline digestion using 1 M NaOH, extraction and purification of the steroid were performed in the same step by means of diphasic dialysis. This technique is a semipermeable-membrane technology developed for the direct extraction of relatively low-molecular-mass analytes. The process was performed by employing acetate buffer to homogenize the digested hair, dichloromethane as the extraction solvent at 37 degrees C, and stirring at 150 rpm for 4 h. The recovery was between 74 and 94%. The detection limit was 0.52 ng/g in hair. To evaluate the validity of the methodology, five animals, approximately 3 months old, received an intramuscular anabolic dose of the drug. The xenobiotic could be detected 7 or 14 days after the treatment (between 2.01 and 23.61 ng/g), and until the end of the study (day 98). No statistical difference between hair color and hair assay outcomes was found.
Authors:
A A Durant; C A Fente; C M Franco; B I Vázquez; A Cepeda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  50     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2002 Jan 
Date Detail:
Created Date:  2002-01-23     Completed Date:  2002-05-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  436-40     Citation Subset:  IM    
Affiliation:
Laboratorio de Higiene e Inspección de Alimentos, Departamento de Química Analítica, Nutrición y Bromatología, Facultad de Veterinaria, Universidad de Santiago de Compostela, Campus de Lugo 27002, Spain.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Drug Residues / analysis
Estradiol Congeners / analysis*
Ethinyl Estradiol / analysis*
Gas Chromatography-Mass Spectrometry / methods*
Hair / chemistry*
Reproducibility of Results
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Estradiol Congeners; 57-63-6/Ethinyl Estradiol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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