| Gas chromatography-mass spectrometric method for metabolic profiling of tobacco leaves. | |
| | |
MedLine Citation:
|
PMID: 21560246 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
A gas chromatography-mass spectrometric method was developed for profiling of tobacco leaves. The differentiation among tobacco leaves planted in two different regions was investigated. Prior to analysis, the extraction solvent formulation was optimized and a combination of water, methanol and acetonitrile with a volume ratio of 3:1:1 was found to be optimal. The reproducibility of the method was satisfactory. Kendall tau-b rank correlation coefficients were equal to 1 (p<0.05) for 82% of the resolved peaks (up to 95% of the overall peak areas), indicating the good response correlation. Forty-four compounds including 9 saccharides, 9 alcohols, 9 amino acids, 16 organic acids and phosphoric acid were identified based on standard compounds. The method was successfully applied for profiling of tobacco leaves from Zimbabwe and Yunnan of China. Our result revealed that levels of saccharides and their derivatives including xylose, ribose, fructose, glucose, turanose, xylitol and glyceric acid were more abundant while sucrose, glucitol and D-gluconic acid were less abundant in tobacco leaves from Yunnan as compared to those from Zimbabwe. Amino acids such as L-alanine, L-tyrosine and L-threonine were found to be richer in Zimbabwe tobacco than in Yunnan tobacco. |
| | |
Authors:
|
Yong Li; Tao Pang; Yanli Li; Xiaolin Wang; Qinghua Li; Xin Lu; Guowang Xu |
Related Documents
:
|
16377446 - Protective effect of s-allylcysteine on 3-nitropropionic acid-induced lipid peroxidatio... 21469696 - Antituberculosis cycloartane triterpenoids from radermachera boniana. 10403836 - Marked expression of glutathione s-transferase a4-4 detoxifying 4-hydroxy-2(e)-nonenal ... 21257776 - Corynebacterium glutamicum as a host for synthesis and export of d-amino acids. 2921596 - Pancytopenia--a rare manifestation of folic acid deficiency. 15997846 - Identification and quantitative determination of eudesmane-type acids from the essentia... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-5-10 |
Journal Detail:
|
Title: Journal of separation science Volume: - ISSN: 1615-9314 ISO Abbreviation: - Publication Date: 2011 May |
Date Detail:
|
Created Date: 2011-5-11 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101088554 Medline TA: J Sep Sci Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Affiliation:
|
CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Qualitative and quantitative analyses of Epimedium wushanense by high-performance liquid chromatogra...
Next Document: Iron oxide nanoparticle coating of organic polymer-based monolithic columns for phosphopeptide enric...