Document Detail


Gas chromatographic-mass spectrometric identification of four triene monoepoxides of arachidonic acid in human plasma.
MedLine Citation:
PMID:  2327589     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Four triene monoepoxides of arachidonic acid have been identified as endogenous components of human plasma, the epoxy groups being in the 5,6-, 8,9-, 11,12- and 14,15-positions. Prior to trimethylsilylation and gas chromatographic-mass spectrometric analysis, both the expoxy and ester functions were reduced to hydroxy groups and the double bonds were hydrogenated catalytically. Saturation of the double bonds gave diagnostic spectra that were suitable for elucidating the position of the epoxy group. The shift in the fragmentation of a deuteriated sample verified the presence of the intact epoxides prior to chemical reduction. The presence of the double bonds in the epoxy molecules was demonstrated by reduction using homogeneous catalysis with tris(triphenylphosphine)rhodium(I) chloride and deuterium.
Authors:
G A Ulsaker; G Teien
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Analyst     Volume:  115     ISSN:  0003-2654     ISO Abbreviation:  Analyst     Publication Date:  1990 Mar 
Date Detail:
Created Date:  1990-05-14     Completed Date:  1990-05-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372652     Medline TA:  Analyst     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  259-62     Citation Subset:  IM    
Affiliation:
Norwegian Medicines Control Authority, Oslo.
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MeSH Terms
Descriptor/Qualifier:
8,11,14-Eicosatrienoic Acid / analogs & derivatives,  blood*
Aluminum
Aluminum Compounds*
Deuterium
Fatty Acids, Unsaturated / blood*
Gas Chromatography-Mass Spectrometry*
Humans
Lithium
Lithium Compounds*
Molecular Structure
Oxidation-Reduction
Chemical
Reg. No./Substance:
0/Aluminum Compounds; 0/Fatty Acids, Unsaturated; 0/Lithium Compounds; 16853-85-3/lithium aluminum hydride; 7324-41-6/8,11,14-Eicosatrienoic Acid; 7429-90-5/Aluminum; 7439-93-2/Lithium; 7782-39-0/Deuterium; 81246-84-6/5,6-epoxy-8,11,14-eicosatrienoic acid; 81246-85-7/8,9-epoxyeicosatrienoic acid; 81276-02-0/11,12-epoxy-5,8,14-eicosatrienoic acid; 81276-03-1/14,15-epoxy-5,8,11-eicosatrienoic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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