Document Detail

Gas-chromatographic determination of cholesterol in serum: candidate reference method.
MedLine Citation:
PMID:  3986996     Owner:  NLM     Status:  MEDLINE    
We present a candidate Reference Method for determination of total cholesterol in serum. The method is based on high-resolution capillary gas chromatography, and we took special precautions with respect to weighing, calibration, and chromatographic peak integration. Analytical recovery of added cholesterol was essentially complete (99.99%, SD 0.48%) and reproducibility was excellent (total CV 0.35-0.50%). When cholesterol was determined in a reference serum certified by means of an established candidate Definitive Method, no significant bias could be detected.
H J Derks; A van Heiningen; H C Koedam
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical chemistry     Volume:  31     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1985 May 
Date Detail:
Created Date:  1985-06-18     Completed Date:  1985-06-18     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  691-4     Citation Subset:  IM    
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MeSH Terms
Chemical Phenomena
Cholesterol / blood*
Chromatography, Gas
Reference Standards
Reg. No./Substance:

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