Document Detail


Gas chromatographic assay for the new antitumor agent sulfamic acid diester (NSC 329680) and its stability in buffer, blood and plasma.
MedLine Citation:
PMID:  3410909     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A sensitive gas chromatographic assay with electron-capture detection has been developed for sulfamic acid diester (sulfamic acid 1,7-heptanediyl ester, NSC 329680) based on its conversion to 1,7-diiodoheptane in the presence of excess sodium iodide. The assay is linear up to 1 microgram/ml sulfamic acid diester and has a lower limit of detection of 25 ng/ml from 0.5 ml plasma. The coefficient of variation of the assay is 6.4% at 1 microgram/ml and 8.0% at 100 ng/ml. Sulfamic acid diester is relatively stable in 0.9% sodium chloride and 0.1 M sodium phosphate buffers, pH 7.0-9.0, with half-lives greater than 38 h. The major breakdown product of sulfamic acid diester is sulfamic acid 1,7-heptane-monoyl ester. When added to whole blood sulfamic acid diester shows concentration-dependent breakdown. At 50 and 100 micrograms/ml sulfamic acid diester, the half-time in whole blood is 6.9 h and 65% of the drug is sequestered by the blood cells. At 10 micrograms/ml sulfamic acid diester in blood, there is no detectable breakdown of the drug over 24 h and all of the drug is sequestered by the blood cells. Protein binding of sulfamic acid diester in human plasma is 82% at 10 micrograms/ml and 68% at 100 micrograms/ml.
Authors:
J I Brodfuehrer; G Powis
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of chromatography     Volume:  427     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1988 Jun 
Date Detail:
Created Date:  1988-10-11     Completed Date:  1988-10-11     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  247-55     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents / analysis*,  blood,  urine
Blood Proteins / analysis
Chromatography, Gas
Erythrocytes / analysis
Humans
Mass Spectrometry
Sulfonic Acids / analysis*,  blood,  urine
Grant Support
ID/Acronym/Agency:
CM67904/CM/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Blood Proteins; 0/Sulfonic Acids; 96892-57-8/hepsulfam

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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