| Gas chromatographic analysis of infant formulas for total fatty acids, including trans fatty acids. | |
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MedLine Citation:
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PMID: 11878624 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Twelve powdered and 13 liquid infant formulas were analyzed by using an extension of AOAC Official Method 996.01 for fat analysis in cereal products. Samples were hydrolyzed with 8 N HCl and extracted with ethyl and petroleum ethers. Fatty acid methyl esters were prepared by refluxing the mixed ether extracts with methanolic sodium hydroxide in the presence of 14% boron trifluoride in methanol. The extracts were analyzed by gas chromatography. In powdered formulas, saturated fatty acid (SFA) content (mean +/- SD; n = 12) was 41.05 +/- 3.94%, monounsaturated fatty acid (MUFA) content was 36.97 +/- 3.38%, polyunsaturated fatty acid (PUFA) content was 20.07 +/- 3.08%, and total trans fatty acid content was 1.30 +/- 1.27%. In liquid formulas, SFA content (mean +/- SD; n = 13) was 42.29 +/- 2.98%, MUFA content was 36.05 +/- 2.47%, PUFA content was 20.65 +/- 2.40%, and total trans fatty acid content was 0.88 +/- 0.54%. Total fat content in powdered formulas ranged from 4.4 to 5.5 g/100 kcal and linoleic acid content ranged from 868 to 1166 mg/100 kcal. In liquid formulas, total fat content ranged from 4.1 to 5.1 g/100 kcal and linoleic acid content ranged from 820 to 1100 mg/100 kcal. There were no significant differences between powdered and liquid infant formulas in concentrations of total fat, SFA, MUFA, PUFA, or trans fatty acids. |
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Authors:
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Subramaniam Satchithanandam; Jan Fritsche; Jeanne I Rader |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of AOAC International Volume: 85 ISSN: 1060-3271 ISO Abbreviation: J AOAC Int Publication Date: 2002 Jan-Feb |
Date Detail:
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Created Date: 2002-03-06 Completed Date: 2002-08-20 Revised Date: 2008-03-17 |
Medline Journal Info:
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Nlm Unique ID: 9215446 Medline TA: J AOAC Int Country: United States |
Other Details:
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Languages: eng Pagination: 86-94 Citation Subset: IM |
Affiliation:
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U.S. Food and Drug Administration, Office of Nutritional Products, Labeling and Dietary Supplements, Washington, DC 20204, USA. ssubrama@cfsan.fda.gov |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Algorithms Calibration Chromatography, Gas Esters / analysis Fatty Acids / analysis* Humans Infant Infant Food / analysis* Methylation Reference Standards Reproducibility of Results Triglycerides / analysis |
| Chemical | |
Reg. No./Substance:
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0/Esters; 0/Fatty Acids; 0/Triglycerides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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