Document Detail

Gas chromatographic analysis of infant formulas for total fatty acids, including trans fatty acids.
MedLine Citation:
PMID:  11878624     Owner:  NLM     Status:  MEDLINE    
Twelve powdered and 13 liquid infant formulas were analyzed by using an extension of AOAC Official Method 996.01 for fat analysis in cereal products. Samples were hydrolyzed with 8 N HCl and extracted with ethyl and petroleum ethers. Fatty acid methyl esters were prepared by refluxing the mixed ether extracts with methanolic sodium hydroxide in the presence of 14% boron trifluoride in methanol. The extracts were analyzed by gas chromatography. In powdered formulas, saturated fatty acid (SFA) content (mean +/- SD; n = 12) was 41.05 +/- 3.94%, monounsaturated fatty acid (MUFA) content was 36.97 +/- 3.38%, polyunsaturated fatty acid (PUFA) content was 20.07 +/- 3.08%, and total trans fatty acid content was 1.30 +/- 1.27%. In liquid formulas, SFA content (mean +/- SD; n = 13) was 42.29 +/- 2.98%, MUFA content was 36.05 +/- 2.47%, PUFA content was 20.65 +/- 2.40%, and total trans fatty acid content was 0.88 +/- 0.54%. Total fat content in powdered formulas ranged from 4.4 to 5.5 g/100 kcal and linoleic acid content ranged from 868 to 1166 mg/100 kcal. In liquid formulas, total fat content ranged from 4.1 to 5.1 g/100 kcal and linoleic acid content ranged from 820 to 1100 mg/100 kcal. There were no significant differences between powdered and liquid infant formulas in concentrations of total fat, SFA, MUFA, PUFA, or trans fatty acids.
Subramaniam Satchithanandam; Jan Fritsche; Jeanne I Rader
Related Documents :
8030974 - Inactivation of visna virus and other enveloped viruses by free fatty acids and monogly...
21248344 - Impact of citric acid and calcium ions on acid solubilization of mechanically separated...
15653514 - Flavor and stability of pasteurized milk with elevated levels of conjugated linoleic ac...
557894 - Breast milk composition in ethiopian and swedish mothers. iii. amino acids and other ni...
21948824 - Metabolome remodeling during the acidogenic-solventogenic transition in clostridium ace...
6529564 - Nonaromatic amino acids in the combining site region of a monoclonal anti-spin-label an...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of AOAC International     Volume:  85     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2002 Jan-Feb
Date Detail:
Created Date:  2002-03-06     Completed Date:  2002-08-20     Revised Date:  2008-03-17    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  86-94     Citation Subset:  IM    
U.S. Food and Drug Administration, Office of Nutritional Products, Labeling and Dietary Supplements, Washington, DC 20204, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Chromatography, Gas
Esters / analysis
Fatty Acids / analysis*
Infant Food / analysis*
Reference Standards
Reproducibility of Results
Triglycerides / analysis
Reg. No./Substance:
0/Esters; 0/Fatty Acids; 0/Triglycerides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Determination of capsaicinoids in salsa by liquid chromatography and enzyme immunoassay.
Next Document:  Determination of low-level glucose and fructose in raw and refined crystalline sugar by high-perform...