Document Detail


A Gas Chromatography-Mass Spectrometry Method for the Determination of Free Amino Acids as their Dimethyl-tert-butylsilyl (TBDMS) Derivatives in Animal Source Food.
MedLine Citation:
PMID:  22356248     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The suitability of a one-step derivatization procedure using N-methyl-N-(tert-butyldimethylsilyl)trifluoroacetamide for the simultaneous assay of 22 free amino acids, and its application for their analysis in six animal source food (pork, dry cured ham, chicken stock, fresh cheese, ripened cheese and dry salted sardine) by GC-MS was studied. All the 22 free amino acid derivatives were correctly detected and resolved. Reproducibility (%RSD) of the method was in the range of 1.9-12.2%. Detection and quantitation limits of the analytical procedure ranged from 0.01 to 0.46 mg/100 g dry weight and from 0.02 to 1.55 mg/100 g dry weight respectively. The calibration curves were linear within the range 0.1-15.0 mg/100 g with correlation coefficient values (R2) from 0.9891 to 0.9983. All analyzed food products showed similar free amino acids contents to those found in the scientific literature. The proposed GC-MS method for the determination of free amino acids in animal source food can be used in routine for both analytical and research purposes. <i></i><i><i>
Authors:
Estefanía Jiménez-Martín; Jorge Ruiz; Trinidad Perez-Palacios; Antonio Silva; Teresa Antequera
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-2-23
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  -     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-2-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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