Document Detail


Gap junctions and connexon hemichannels in human embryonic stem cells.
MedLine Citation:
PMID:  16574755     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Intercellular communication via gap junctions is thought to play an important role in embryonic cell survival and differentiation. Classical studies demonstrated both dye and electrical coupling of cells in the inner cell mass of mouse embryos, as well as the development of restrictions against coupling between cells of the inner cell mass and surrounding trophectoderm. Here we demonstrate extensive gap junctional communication between human embryonic stem (ES) cells, the pluripotent cells isolated from the inner cell mass of preimplantation blastocysts. Human ES cells maintained in vitro expressed RNA for 18 of the 20 known connexins; only connexin 40.1 (Cx40.1) and Cx50 were not detected by reverse transcription-polymerase chain reaction. Cx40, Cx43, and Cx45 were visualized by immunofluorescence at points of contact between adjacent cells. Electron microscopy confirmed that neighboring cells formed zones of tight membrane apposition characteristic of gap junctions. Fluorescent dye injections demonstrated extensive coupling within human ES cell colonies growing on mouse embryonic fibroblast (MEF) feeder cells, whereas dye coupling between human ES cells and adjacent MEFs was extremely rare. Physiological recordings demonstrated electrical and dye coupling between human ES cells in feeder-free monolayers and between isolated human ES cell pairs. Octanol, 18-alpha-glycyrrhetinic acid, and arylaminobenzoates inhibited transjunctional currents. Dye uptake studies on human ES cell monolayers and recordings from solitary human ES cells gave evidence for the surface expression of connexon hemichannels. Human ES cells provide a unique system for the study of human connexin proteins and their potential functions in cellular differentiation and the maintenance of pluripotency.
Authors:
James E Huettner; Aiwu Lu; Yun Qu; Yingji Wu; Mijeong Kim; John W McDonald
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2006-03-30
Journal Detail:
Title:  Stem cells (Dayton, Ohio)     Volume:  24     ISSN:  1066-5099     ISO Abbreviation:  Stem Cells     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-07-06     Completed Date:  2006-09-27     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9304532     Medline TA:  Stem Cells     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1654-67     Citation Subset:  IM    
Affiliation:
Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri 63110, USA . huettner@cellbio.wustl.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Communication / physiology
Cell Count
Connexins / metabolism,  physiology*
Dye Dilution Technique
Electric Capacitance
Electric Conductivity
Embryo, Mammalian / cytology*
Gap Junctions / physiology*
Humans
Ion Channels / drug effects,  physiology*
Membrane Potentials / drug effects
Mice
Stem Cells / physiology*
Grant Support
ID/Acronym/Agency:
NS045023/NS/NINDS NIH HHS; NS39577/NS/NINDS NIH HHS; NS40520/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Connexins; 0/Ion Channels

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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