| Gap junctional remodeling by hypoxia in cultured neonatal rat ventricular myocytes. | |
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MedLine Citation:
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PMID: 15769449 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: Altered gap junctional coupling of ventricular myocytes plays an important role in arrhythmogenesis in ischemic heart disease. Since hypoxia is a major component of ischemia, we tested the hypothesis that hypoxia causes gap junctional remodeling accompanied by conduction disturbances. METHODS: Cultured neonatal rat ventricular myocytes were exposed to hypoxia (1% O(2)) for 15 min to 5 h, connexin43 (Cx43) expression was analyzed, and conduction velocity was measured using the Micro-Electrode Array data acquisition system. RESULTS: After 15 min of hypoxia, conduction velocity was unaffected, while total Cx43, including the phosphorylated and nonphosphorylated isoforms, was increased. After 5 h of hypoxia, total Cx43 protein was decreased by 50%, while the nonphosphorylated Cx43 isoform was unchanged. Confocal analyses yielded a 55% decrease in the gap junctional Cx43 fluorescence signal, a 55% decrease in gap junction number, and a 26% decrease in size. The changes in Cx43 were not accompanied by changes in mRNA levels. The reduction in Cx43 protein levels was associated with a approximately 20% decrease in conduction velocity compared to normoxic cultures. CONCLUSIONS: Short-term hypoxia (5 h) decreases Cx43 protein and conduction velocity, thereby contributing to the generation of an arrhythmogenic substrate. |
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Authors:
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Naama Zeevi-Levin; Yaron D Barac; Yotam Reisner; Irina Reiter; Gal Yaniv; Gideon Meiry; Zaid Abassi; Sawa Kostin; Jutta Schaper; Michael R Rosen; Nitzan Resnick; Ofer Binah |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Cardiovascular research Volume: 66 ISSN: 0008-6363 ISO Abbreviation: Cardiovasc. Res. Publication Date: 2005 Apr |
Date Detail:
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Created Date: 2005-03-16 Completed Date: 2005-08-05 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0077427 Medline TA: Cardiovasc Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 64-73 Citation Subset: IM |
Affiliation:
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Rappaport Faculty of Medicine, Technion, Haifa, Israel. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Anoxia / metabolism* Arrhythmias, Cardiac / metabolism*, physiopathology Blotting, Western / methods Connexin 43 / analysis, genetics, metabolism Gap Junctions / chemistry, metabolism* Heart Conduction System Heart Ventricles Immunohistochemistry / methods Microscopy, Confocal Myocytes, Cardiac / chemistry, metabolism* RNA, Messenger / analysis Rats Rats, Sprague-Dawley Reverse Transcriptase Polymerase Chain Reaction Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Connexin 43; 0/RNA, Messenger |
| Comments/Corrections | |
Comment In:
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Cardiovasc Res. 2005 Apr 1;66(1):9-11
[PMID:
15769443
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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