Document Detail

Gap junctional intercellular communication of primary and asbestos-associated malignant human mesothelial cells.
MedLine Citation:
PMID:  8394779     Owner:  NLM     Status:  MEDLINE    
We examined gap junctional intercellular communication (GJIC) of primary human mesothelial cells and cell lines of asbestos-associated human pleural mesotheliomas, and the effect of asbestos and other mineral fibres on these cells. In homologous cultures, the GJIC capacity of six out of seven tumour cell lines was markedly less than for primary mesothelial cells. This defect in GJIC appeared not to be at the expression level of mRNA and protein of the gene encoding the 43 kDa gap junction protein. In heterologous cocultures of tumour cells and primary mesothelial cells, however, 80-90% of the tumour cell/normal cell contacts were functional. Exposure of primary mesothelial cells to TPA, a phorbol ester tumour promoter, resulted in marked inhibition of GJIC, being an action common to numerous tumour promoters. Such an effect though was not observed with the carcinogenic mesothelioma-inducing mineral fibres chrysotile and amosite, neither with glass wool. These results suggest that a permanent defect in GJIC capacity is a common feature of human mesothelioma cells, but how mineral fibres are involved in the process of mesotheliomagenesis is still unclear.
K Linnainmaa; K Pelin; E Vanhala; T Tuomi; C Piccoli; D J Fitzgerald; H Yamasaki
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Carcinogenesis     Volume:  14     ISSN:  0143-3334     ISO Abbreviation:  Carcinogenesis     Publication Date:  1993 Aug 
Date Detail:
Created Date:  1993-09-21     Completed Date:  1993-09-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8008055     Medline TA:  Carcinogenesis     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1597-602     Citation Subset:  IM    
Institute of Occupational Health, Department of Industrial Hygiene, Helsinki, Finland.
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MeSH Terms
Asbestos / adverse effects*
Cell Communication / drug effects*,  physiology*
Cells, Cultured
Epithelial Cells
Epithelium / drug effects,  physiology
Gene Expression / drug effects,  genetics
Intercellular Junctions / drug effects*,  physiology*
Membrane Proteins / genetics
Mesothelioma / etiology,  genetics,  pathology*
Pleural Neoplasms / etiology,  genetics,  pathology*
Tetradecanoylphorbol Acetate / toxicity
Tumor Cells, Cultured
Grant Support
Reg. No./Substance:
0/Connexins; 0/Membrane Proteins; 1332-21-4/Asbestos; 16561-29-8/Tetradecanoylphorbol Acetate

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