Document Detail

Gap junctional intercellular communication of cultured rat liver parenchymal cells is stabilized by epithelial cells and their isolated plasma membranes.
MedLine Citation:
PMID:  8125169     Owner:  NLM     Status:  MEDLINE    
The gap junctional intercellular communication (GJIC) determined by measuring dye coupling with Lucifer yellow, decreased within 3 d from 66% to 28% in monocultures of rat liver parenchymal cells. Coculturing of the parenchymal cells with a nonparenchymal epithelial cell line from rat liver resulted in increased and stabilized intercellular communication (83% after 3 d). The presence of isolated plasma membrane vesicles of the nonparenchymal epithelial cells also stabilized the intercellular communication between the liver parenchymal cells (70% after 3 d). When liver parenchymal cells were cocultured with a rat liver fibroblast cell line the gap junctional communication between the parenchymal cells was not stabilized (43% after 3 d), and isolated plasma membrane vesicles of the fibroblast were also unable to support the GJIC in parenchymal cells (35% after 3 d). It is concluded that plasma membrane constituents of the nonparenchymal epithelial cells were responsible for the stabilization of the GJIC between parenchymal cells. A heterotypic gap junctional communication between parenchymal and nonparenchymal cells was not observed.
B Diener; N Beer; H Dürk; M Traiser; D Utesch; R J Wieser; F Oesch
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experientia     Volume:  50     ISSN:  0014-4754     ISO Abbreviation:  Experientia     Publication Date:  1994 Feb 
Date Detail:
Created Date:  1994-04-08     Completed Date:  1994-04-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376547     Medline TA:  Experientia     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  124-6     Citation Subset:  IM    
Institut für Toxikologie, Universität Mainz, Germany.
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MeSH Terms
Cell Communication*
Cell Membrane / physiology
Cells, Cultured
Epithelial Cells
Gap Junctions / physiology*
Liver / cytology*
Rats, Sprague-Dawley

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