| Gap junction protein phenotypes of the human heart and conduction system. | |
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MedLine Citation:
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PMID: 8542077 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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INTRODUCTION: Gap junction channels are major determinants of intercellular resistance to current flow between cardiac myocytes. Alterations in gap junctions may contribute to development of arrhythmia substrates in patients. However, there is significant interspecies variation in the types and amounts of gap junction subunit proteins (connexins) expressed in disparate regions of mammalian hearts. To elucidate determinants of conduction properties in the human heart, we characterized connexin phenotypes of specific human cardiac tissues with different conduction properties. METHODS AND RESULTS: The distribution and relative abundance of Cx37, Cx40, Cx43, Cx45, and Cx46 were studied immunohistochemically using monospecific antibodies and frozen sections of the sinoatrial node and adjacent atria. AV node and His bundle, the bundle branches, and the left and right ventricular walls. Patterns of expression of these connexins in the human heart differed from those in previous animal studies. Sinus node gap junctions were small and sparse and contained Cx45 and apparently smaller amounts of Cx40 but no Cx43. AV node gap junctions were also small and contained mainly Cx45 and Cx40 but, unlike the sinus node, also expressed Cx43. Atrial gap junctions were larger than nodal junctions and contained moderate amounts of Cx40, Cx43, and Cx45. Junctions in the bundle branches were the largest in size and contained abundant amounts of Cx40, Cx43, and Cx45. Gap junctions in ventricular myocardium contained mainly Cx43 and Cx45; only a very small and amount of ventricular Cx40 was detected in subendocardial myocyte junctions and endothelial cells of small to medium sized intramural coronary arteries. Minimal Cx37 and Cx46 immunoreactivity was detected between occasional atrial or ventricular myocytes. CONCLUSIONS: The relative amounts of individual connexins and the number and size of gap junctions vary greatly in specific regions of the human heart with different conduction properties. These differences likely play a role in regulating cardiac conduction velocity. Differences in the connexin phenotypes of specific regions of the human heart and experimental animal hearts must be considered in future experimental or modeling studies of cardiac conduction. |
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Authors:
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L M Davis; M E Rodefeld; K Green; E C Beyer; J E Saffitz |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of cardiovascular electrophysiology Volume: 6 ISSN: 1045-3873 ISO Abbreviation: J. Cardiovasc. Electrophysiol. Publication Date: 1995 Oct |
Date Detail:
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Created Date: 1996-02-13 Completed Date: 1996-02-13 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9010756 Medline TA: J Cardiovasc Electrophysiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 813-22 Citation Subset: IM |
Affiliation:
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Department of Pediatrics, Washington University School of Medicine, St. Louis, Missouri 63110, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Connexins / genetics, metabolism* Gap Junctions / metabolism* Heart Conduction System / metabolism* Humans Immunohistochemistry Microscopy, Electron Middle Aged Myocardium / metabolism* Phenotype Tissue Distribution |
| Grant Support | |
ID/Acronym/Agency:
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HL17646/HL/NHLBI NIH HHS; HL45466/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Connexins |
| Comments/Corrections | |
Comment In:
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J Cardiovasc Electrophysiol. 1996 Apr;7(4):382-3
[PMID:
8777487
]
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Erratum In:
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J Cardiovasc Electrophysiol 1996 Apr;7(4):383-5 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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