| Ganoderiol F, a ganoderma triterpene, induces senescence in hepatoma HepG2 cells. | |
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MedLine Citation:
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PMID: 16635496 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ganoderiol F (GolF), a tetracyclic triterpene, was isolated from Ganoderma amboinense and found to induce senescence of cancer cell lines. GolF induced growth arrest of cancer cell lines HepG2, Huh7 and K562, but exerted much less effect in hepatoma Hep3B cells and normal lung fibroblast MRC5 cells, and no effect on peripheral blood mononuclear cells. GolF treatment of the cancer cells, with the exception of Hep3B, resulted in prompt inhibition of DNA synthesis and arrest of cell progression cycle in G1 phase. Short-term exposure of HepG2 cells to GolF temporarily arrested progression of the cell cycle; cell growth was recovered if the drug was withdrawn from the medium after a 24-h exposure. After 18 days of continuous treatment of HepG2 cells with 30 muM GolF, over 50% of cells were found to be enlarged and flattened, and were beta-galactosidase positive phenotypes of senescent cells. GolF was found to inhibit activity of topoisomerases in vitro, which may contribute to the inhibition of cellular DNA synthesis. Activation of the mitogen-activated protein kinase EKR and up-regulation of cyclin-dependent kinase inhibitor p16 were found in early stages of GolF treatment and were presumed to cause cell-cycle arrest and trigger premature senescence of HepG2 cells. The growth-arrest and senescence induction capability on cancer cells suggest anticancer potential of GolF. |
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Authors:
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Ue-Min Chang; Chyi-Hann Li; Liang-In Lin; Cheng-Po Huang; Lou-Sing Kan; Shwu-Bin Lin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-03-28 |
Journal Detail:
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Title: Life sciences Volume: 79 ISSN: 0024-3205 ISO Abbreviation: Life Sci. Publication Date: 2006 Aug |
Date Detail:
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Created Date: 2006-08-04 Completed Date: 2006-09-13 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0375521 Medline TA: Life Sci Country: England |
Other Details:
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Languages: eng Pagination: 1129-39 Citation Subset: IM |
Affiliation:
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Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, Taiwan ROC. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antibiotics, Antineoplastic
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pharmacology* Blotting, Western Carcinoma, Hepatocellular / pathology* Cell Aging / drug effects* Cell Line, Tumor Cell Proliferation / drug effects Cyclin-Dependent Kinase Inhibitor p16 / biosynthesis DNA Topoisomerases, Type I / metabolism DNA, Neoplasm / biosynthesis, metabolism Enzyme Activation / physiology Flow Cytometry G1 Phase / drug effects Ganoderma / chemistry* Humans Liver Neoplasms / pathology* Mitogen-Activated Protein Kinases / metabolism Reverse Transcriptase Polymerase Chain Reaction Triterpenes / pharmacology* Tumor Suppressor Proteins / biosynthesis |
| Chemical | |
Reg. No./Substance:
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0/Antibiotics, Antineoplastic; 0/Cyclin-Dependent Kinase Inhibitor p16; 0/DNA, Neoplasm; 0/Triterpenes; 0/Tumor Suppressor Proteins; 0/ganoderiol F; EC 2.7.11.24/Mitogen-Activated Protein Kinases; EC 5.99.1.2/DNA Topoisomerases, Type I |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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