Document Detail

Ganoderic acid Mf and S induce mitochondria mediated apoptosis in human cervical carcinoma HeLa cells.
MedLine Citation:
PMID:  21036023     Owner:  NLM     Status:  MEDLINE    
In this work, the effects of a pair of positional isomer of ganoderic acids (GAs), namely ganoderic acid Mf (GA-Mf) and ganoderic acid S (GA-S) purified from the fermented mycelia of Ganoderma lucidum, on induction of cell apoptosis and the apoptotic pathway in HeLa cells were investigated. The results demonstrate that both isomers decreased cell population growth on various human carcinoma cell lines by MTT assay, while GA-Mf had better selectivity between normal and cancer cells. The flow cytometry analysis indicated that treatment of HeLa cells with GA-S caused cell cycle arrest in the S phase, while GA-Mf caused cell cycle arrest in the G1 phase. Compared with GA-S, GA-Mf had more potent increase in the number of early and late apoptotic cells. Treatment of HeLa cells with each isomer decreased the mitochondria membrane potential and caused the release of cytochrome c from mitochondria into the cytosol. In addition, stimulation of caspase-3 and caspase-9 activity was observed. The Bax/Bcl-2 ratio was also increased in GA-treated HeLa cells. The results demonstrated that both isomers GA-Mf and GA-S induced apoptosis of human HeLa cells through a mitochondria mediated pathway, but they had the different cell cycle arrest specificity. The findings will be helpful to the development of useful cancer chemopreventive compounds from G. lucidum.
Ru-Ming Liu; Jian-Jiang Zhong
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-10-29
Journal Detail:
Title:  Phytomedicine : international journal of phytotherapy and phytopharmacology     Volume:  18     ISSN:  1618-095X     ISO Abbreviation:  Phytomedicine     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-03-18     Completed Date:  2012-05-17     Revised Date:  2012-06-08    
Medline Journal Info:
Nlm Unique ID:  9438794     Medline TA:  Phytomedicine     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  349-55     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier GmbH. All rights reserved.
State Key Laboratory of Bioreactor Engineering, School of Bioengineering, East China University of Science and Technology, Shanghai, China.
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MeSH Terms
Apoptosis / drug effects*
Carcinoma / genetics,  pathology*,  prevention & control
Caspase 3 / antagonists & inhibitors,  metabolism
Caspase 9 / antagonists & inhibitors,  metabolism
Cell Line
Cytochromes c / metabolism
Dose-Response Relationship, Drug
G1 Phase / drug effects
Gene Expression Regulation, Neoplastic / drug effects
HeLa Cells
Membrane Potential, Mitochondrial / drug effects
Mitochondria / metabolism*,  pathology
Mycelium / chemistry
Reishi / chemistry*
S Phase / drug effects
Triterpenes / isolation & purification,  pharmacology*
Uterine Cervical Neoplasms / genetics,  pathology*,  prevention & control
Reg. No./Substance:
0/Triterpenes; 0/ganoderic acid Mf; 0/ganoderic acid S; 9007-43-6/Cytochromes c; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 9
Erratum In:
Phytomedicine. 2012 Apr 15;19(6):569

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