Document Detail


Gangliosides from human granulocytes: a nano-ESI QTOF mass spectrometry fucosylation study of low abundance species in complex mixtures.
MedLine Citation:
PMID:  11506229     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nano-ESI QTOF MS was used for sensitive mapping and sequencing of single molecular species in complex ganglioside mixtures obtained from human granulocytes, where the fucosylated carbohydrate chains of granulocyte gangliosides carry sLex and VIM-2 epitopes postulated to interact with E-selectin of the blood vessel wall in the early phase of the inflammation process. Functionally relevant components are expressed only at a low level, but using the negative ion detection it is possible to trace and identify such species, which were not detectable even by TLC. Advantage of the low-energy CID fragmentation for low abundance components of the complex ganglioside mixtures in the negative ion mode is to produce clear-cut series of fragment ions for sequencing. Fucosylation analysis carried out for each molecular species by MS/MS permits the clear distinction between sLex and VIM-2 epitope. VIM-2 epitope was expressed in all species with a longer sugar core, while in the short oligosaccharide chain with a sLex only, using biological material at a mid-femtomol level detection.
Authors:
W Metelmann; J Peter-Katalinić; J Müthing
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of the American Society for Mass Spectrometry     Volume:  12     ISSN:  1044-0305     ISO Abbreviation:  J. Am. Soc. Mass Spectrom.     Publication Date:  2001 Aug 
Date Detail:
Created Date:  2001-08-16     Completed Date:  2001-08-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9010412     Medline TA:  J Am Soc Mass Spectrom     Country:  United States    
Other Details:
Languages:  eng     Pagination:  964-73     Citation Subset:  IM    
Affiliation:
Medical Faculty, Institute for Medical Physics and Biophysics, University of Münster, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain
Carbohydrate Sequence
Carbohydrates / analysis
Cattle
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
G(M1) Ganglioside / chemistry
Gangliosides / analysis*,  chemistry
Granulocytes / chemistry*
Humans
Molecular Structure
Oligosaccharides / chemistry
Sequence Analysis / instrumentation,  methods
Spectrometry, Mass, Electrospray Ionization
Chemical
Reg. No./Substance:
0/Carbohydrates; 0/Gangliosides; 0/Oligosaccharides; 0/VIM-2 determinant; 12707-58-3/ganglioside, GD1a; 19553-76-5/ganglioside, GD1b; 37758-47-7/G(M1) Ganglioside

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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