| Gamma irradiation induces acetylcholine-evoked, endothelium-independent relaxation and activates K-channels of isolated pulmonary artery of rats. | |
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MedLine Citation:
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PMID: 15590185 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To test the effects of irradiation (R*) on the pulmonary artery (PA). METHODS AND MATERIALS: Isolated PA rings were submitted to gamma irradiation (cesium, 8 Gy/min(-1)) at doses of 20 Gy-140 Gy. Rings were placed in an organ chamber, contracted with serotonin (10(-4) M 5-hydroxytryptamine [5-HT]), then exposed to acetylcholine (ACh) in incremental concentrations. Smooth muscle cell (SMC) membrane potential was measured with microelectrodes. RESULTS: A high dose of irradiation (60 Gy) increased 5HT contraction by 20%, whereas lower (20 Gy) doses slightly decreased it compared with control. In the absence of the endothelium, 5-HT precontracted rings exposed to 20 Gy irradiation developed a dose-dependent relaxation induced by acetylcholine (EI-ACh) with maximal relaxation of 60 +/- 17% (n = 13). This was totally blocked by L-NAME (10(-4) M), partly by 7-nitro indazole; it was abolished by hypoxia and iberiotoxin, decreased by tetra-ethyl-ammonium, and not affected by free radical scavengers. In irradiated rings, hypoxia induced a slight contraction which was never observed in control rings. No differences in SMC membrane potential were observed between irradiated and nonirradiated PA rings. CONCLUSION: Irradiation mediates endothelium independent relaxation by a mechanism involving the nitric oxide pathway and K-channels. |
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Authors:
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Veronique Eder; Mathieu Gautier; Julien Boissière; Catherine Girardin; Manuel Rebocho; Pierre Bonnet |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: International journal of radiation oncology, biology, physics Volume: 60 ISSN: 0360-3016 ISO Abbreviation: Int. J. Radiat. Oncol. Biol. Phys. Publication Date: 2004 Dec |
Date Detail:
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Created Date: 2004-12-13 Completed Date: 2005-01-10 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 7603616 Medline TA: Int J Radiat Oncol Biol Phys Country: United States |
Other Details:
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Languages: eng Pagination: 1530-7 Citation Subset: IM |
Affiliation:
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Laboratoire de Physiopathologie de la Paroi Artérielle, Faculté de Médecine, Université François Rabelais, Tours, France. eder@med.univ-tours.fr |
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetylcholine
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pharmacology* Animals Cesium Endothelium, Vascular / drug effects, radiation effects* Enzyme Inhibitors / pharmacology Gamma Rays* Muscle, Smooth, Vascular / drug effects, radiation effects Nitric Oxide Synthase / antagonists & inhibitors Potassium Channels / physiology, radiation effects* Pulmonary Artery / drug effects, radiation effects* Rats Serotonin / pharmacology Vasoconstriction / drug effects, radiation effects Vasodilation* |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 0/Potassium Channels; 50-67-9/Serotonin; 51-84-3/Acetylcholine; 7440-46-2/Cesium; EC 1.14.13.39/Nitric Oxide Synthase |
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