Document Detail


Gamma-irradiation effects on biopharmaceutical properties of PLGA microspheres loaded with SPf66 synthetic vaccine.
MedLine Citation:
PMID:  18280123     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Gamma-irradiation is currently the method of choice for terminal sterilization of drug delivery systems made from biodegradable polymers. However, the consequences of gamma-sterilization on the immune response induced by microencapsulated antigens have not yet been reported in the literature. The aim of the present work was to evaluate the effect of gamma-irradiation on the biopharmaceutical properties of PLGA microspheres containing SPf66 malarial antigen. Microspheres were prepared by a (w/o/w) double emulsion/solvent extraction method. Once prepared, part of the formulation was irradiated at a dose of 25 kGy using 60Co gamma as radiation source. The in vitro results obtained showed that the gamma-irradiation exposure had no apparent effect on SPf66 integrity and formulation properties such us morphology, size and peptide loading. Only the release rate of SPf66 was slightly faster after gamma-irradiation. Subcutaneous administration of irradiated and non-irradiated microspheres into mice induced a similar immune response (IgG, IgG1, IgG2a levels) and was comparable to that obtained with SPf66 emulsified with Freund's complete adjuvant. These observations illustrate the applicability of gamma-irradiation as a method of terminal sterilization of microparticulate delivery systems based on chemically synthesized antigens encapsulated into biodegradable PLGA microspheres.
Authors:
Manoli Igartua; Rosa M A Hernández; Jaiver Eduardo Rosas; Manuel Elkin Patarroyo; José Luís Pedraz
Related Documents :
17965583 - Gamma-globulin inhibits superantigen-induced lymphocyte proliferation and cytokine prod...
3117613 - Production of lymphokines by hbsag-reactive human t cell clones upon antigenic stimulat...
18832003 - Naturally acquired humoral and cellular immune responses to plasmodium vivax merozoite ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-12-25
Journal Detail:
Title:  European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft für Pharmazeutische Verfahrenstechnik e.V     Volume:  69     ISSN:  0939-6411     ISO Abbreviation:  Eur J Pharm Biopharm     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-19     Completed Date:  2008-08-29     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9109778     Medline TA:  Eur J Pharm Biopharm     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  519-26     Citation Subset:  IM    
Affiliation:
University of the Basque Country (UPV-EHU), Vitoria-Gasteiz, Spain.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Vaccines / administration & dosage*,  chemistry
Biopharmaceutics
Calorimetry, Differential Scanning
Chemistry, Pharmaceutical
Chemistry, Physical
Drug Compounding
Excipients
Female
Gamma Rays
Immunization
Lactic Acid / chemistry*,  radiation effects*
Mice
Mice, Inbred BALB C
Microscopy, Electron, Scanning
Microspheres*
Physicochemical Phenomena
Polyglycolic Acid / chemistry*,  radiation effects*
Protozoan Proteins / administration & dosage*,  chemistry
Recombinant Proteins / administration & dosage*,  chemistry
Vaccines, Synthetic / administration & dosage,  chemistry
Chemical
Reg. No./Substance:
0/Bacterial Vaccines; 0/Excipients; 0/Protozoan Proteins; 0/Recombinant Proteins; 0/SPf66 protein, Plasmodium; 0/Vaccines, Synthetic; 0/polylactic acid-polyglycolic acid copolymer; 26009-03-0/Polyglycolic Acid; 50-21-5/Lactic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Using biorelevant dissolution to obtain IVIVC of solid dosage forms containing a poorly-soluble mode...
Next Document:  Commentary to the article "Human skin penetration of the major components of Australian tea tre...