| Gamma-crosslinked collagen gel without fibrils: analysis of structure and heat stability. | |
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MedLine Citation:
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PMID: 19734687 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This paper reports an analysis of the structure and heat stability of two different collagen gels: conventional collagen gel (neutral gel) and gel without collagen fibrils (acidic gel), previously reported. We performed differential scanning calorimetry (DSC), observations by scanning electron microscope (SEM), observations by atomic force microscope (AFM), and small angle X-ray scattering (SAXS). Collagen fibrils were clearly observed in the neutral gel but not in the acidic gel by both SEM and AFM. A clear endothermic peak was observed at 53-55 degrees Celsius, representing disassembly of collagens in collagen fibrils in the neutral gel but not in the acidic gel. Only a small broad endothermic peak, at 35-43 degrees Celsius, representing the deformation of the triple helical structure of collagen, was observed in the acidic gel. The SAXS pattern also suggested that the neutral gel had a more heterogeneous structure than the acidic gel. The experimental results described here are compatible with the model proposed in a previous paper, and indicate more clearly that the acidic gel has no collagen fibrils and has a different molecular assembly state of Type I collagen than the neutral gel. |
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Authors:
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Naoki Koshimizu; Masahiko Bessho; Shiho Suzuki; Yoshiaki Yuguchi; Shinichi Kitamura; Masayuki Hara |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-09-07 |
Journal Detail:
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Title: Bioscience, biotechnology, and biochemistry Volume: 73 ISSN: 1347-6947 ISO Abbreviation: Biosci. Biotechnol. Biochem. Publication Date: 2009 Sep |
Date Detail:
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Created Date: 2009-09-24 Completed Date: 2009-12-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9205717 Medline TA: Biosci Biotechnol Biochem Country: Japan |
Other Details:
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Languages: eng Pagination: 1915-21 Citation Subset: IM |
Affiliation:
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Department of Biological Science, Graduate School of Science, Osaka Prefecture University, 1-2 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8570, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Calorimetry, Differential Scanning Collagen / chemistry* Hot Temperature* Microscopy, Atomic Force Microscopy, Electron, Scanning Protein Conformation Scattering, Radiation |
| Chemical | |
Reg. No./Substance:
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9007-34-5/Collagen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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