Document Detail

Gamma-aminobutyric acid-induced calcium signalling in rat superior collicular neurones.
MedLine Citation:
PMID:  12435476     Owner:  NLM     Status:  MEDLINE    
Ionotropic gamma-aminobutyric acid (GABA) receptors are known to mediate excitation in neonatal neurones as a crucial developmental factor. In the present study we employed calcium imaging techniques with the calcium indicator Fura-2-AM to study the pharmacology of GABA-induced calcium responses in cultures prepared from neonatal rat superficial superior colliculus (SC), after immunocytochemical labelling confirmed the presence of GABA(C) rho(1) subunits in 35% of neurones. Rises in neuronal intracellular calcium were obtained in response to GABA and also to the subtype-specific GABA(A) agonist 4,5,6,7-tetrahydroisoxazolo[5,4-c]pyridin-3-ol. However, the GABA(C) agonist cis-4-aminocrotonic acid induced calcium response only at unspecifically high concentrations (500 microM). Co-application of GABA antagonists revealed that both GABA(A&C) agonists' actions could be blocked by the GABA(A) antagonist bicuculline but not the GABA(C) antagonists 1,2,5,6-tetrahydro-(pyridin-4-yl) methylphosphinic acid. This suggests that activation of GABA(A) but not GABA(C) receptors contributes to excitatory GABA responses and related calcium signals in neonatal SC neurones.
Anne-Marie White; Bettina Platt
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Neuroscience letters     Volume:  334     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2002-11-18     Completed Date:  2003-01-24     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  79-82     Citation Subset:  IM    
Copyright Information:
Copyright 2002 Elsevier Science Ireland Ltd.
Department of Biomedical Sciences, Institute of Medical Sciences, Foresterhill, University of Aberdeen, Aberdeen, AB25 2ZD, Scotland, UK.
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MeSH Terms
Bicuculline / pharmacology
Calcium / metabolism*
Cells, Cultured
Cytarabine / analogs & derivatives*,  pharmacology
GABA Agonists
GABA Antagonists / pharmacology
Isoxazoles / pharmacology
Neurons / drug effects,  metabolism*
Phosphinic Acids / pharmacology
Pyridines / pharmacology
Receptors, GABA / drug effects,  metabolism*
Superior Colliculi / drug effects,  metabolism
gamma-Aminobutyric Acid / drug effects,  metabolism*
Reg. No./Substance:
0/(1,2,5,6-tetrahydropyridin-4-yl)methylphosphinic acid; 0/GABA Agonists; 0/GABA Antagonists; 0/Isoxazoles; 0/Phosphinic Acids; 0/Pyridines; 0/Receptors, GABA; 113737-52-3/N(4)-chloroacetylcytosine arabinoside; 147-94-4/Cytarabine; 485-49-4/Bicuculline; 56-12-2/gamma-Aminobutyric Acid; 64603-91-4/4,5,6,7-tetrahydroisoxazolo(5,4-c)pyridin-3-ol; 7440-70-2/Calcium

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