| Gallstones play a significant role in Salmonella spp. gallbladder colonization and carriage. | |
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MedLine Citation:
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PMID: 20176950 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Salmonella enterica serovar Typhi can colonize the gallbladder and persist in an asymptomatic carrier state that is frequently associated with the presence of gallstones. We have shown that salmonellae form bile-mediated biofilms on human gallstones and cholesterol-coated surfaces in vitro. Here, we test the hypothesis that biofilms on cholesterol gallbladder stones facilitate typhoid carriage in mice and men. Naturally resistant (Nramp1(+/+)) mice fed a lithogenic diet developed cholesterol gallstones that supported biofilm formation during persistent serovar Typhimurium infection and, as a result, demonstrated enhanced fecal shedding and enhanced colonization of gallbladder tissue and bile. In typhoid endemic Mexico City, 5% of enrolled cholelithiasis patients carried serovar Typhi, and bacterial biofilms could be visualized on gallstones from these carriers whereas significant biofilms were not detected on gallstones from Escherichia coli infected gallbladders. These findings offer direct evidence that gallstone biofilms occur in humans and mice, which facilitate gallbladder colonization and shedding. |
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Authors:
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Robert W Crawford; Roberto Rosales-Reyes; María de la Luz Ramírez-Aguilar; Oscar Chapa-Azuela; Celia Alpuche-Aranda; John S Gunn |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-02-22 |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 107 ISSN: 1091-6490 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-03-03 Completed Date: 2010-04-16 Revised Date: 2010-12-17 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
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Languages: eng Pagination: 4353-8 Citation Subset: IM |
Affiliation:
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Department of Molecular Virology, Center for Microbial Interface Biology, The Ohio State University, Columbus, OH 43210, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biofilms Carrier State Cation Transport Proteins / genetics, physiology Cholesterol / metabolism Feces / microbiology Gallstones / metabolism, microbiology* Humans Mice Microscopy, Electron, Scanning Salmonella typhimurium / growth & development* |
| Grant Support | |
ID/Acronym/Agency:
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AI066208/AI/NIAID NIH HHS; R01 AI066208-05/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cation Transport Proteins; 0/natural resistance-associated macrophage protein 1; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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