| Gallic acid inhibits ribonucleotide reductase and cyclooxygenases in human HL-60 promyelocytic leukemia cells. | |
| | |
MedLine Citation:
|
PMID: 16488533 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Gallic acid (GA) is a naturally occurring polyhydroxyphenolic compound and an excellent free radical scavenger. In this study, we examined its cytotoxic and biochemical effects on the human HL-60 promyelocytic leukemia cell line. GA caused a significant imbalance of deoxynucleosidetriphosphate (dNTP) pool sizes, indicating ribonucleotide reductase inhibition. Moreover, GA induced dose-dependent apoptosis in HL-60 cells (80microM GA led to the induction of apoptosis in 39% of cells) and attenuated progression from G0/G1 to the S phase of the cell cycle (60microM GA doubled the number of cells in G0/G1 phase from 22 to 44% when compared to untreated controls). We further determined IC(50) values of 3.5 and 4.4nM for the inhibition of cyclooxygenases I and II, respectively. When cells were simultaneously treated with GA and trimidox, another inhibitor of RR, highly synergistic growth inhibitory effects could be observed. Taken together, we identified novel biochemical effects of GA which could be the basis for further preclinical and in vivo studies. |
| | |
Authors:
|
Sibylle Madlener; Christoph Illmer; Zsuzsanna Horvath; Philipp Saiko; Annemarie Losert; Irene Herbacek; Michael Grusch; Howard L Elford; Georg Krupitza; Astrid Bernhaus; Monika Fritzer-Szekeres; Thomas Szekeres |
Related Documents
:
|
19384733 - An extract from ricinus communis l. leaves possesses cytotoxic properties and induces a... 10552963 - Defective apoptotic signal transduction pathway downstream of caspase-3 in human b-lymp... 12096823 - Secondary cell wall deposition causes radial growth of fibre cells in the maturation zo... |
Publication Detail:
|
Type: Journal Article Date: 2006-02-20 |
Journal Detail:
|
Title: Cancer letters Volume: 245 ISSN: 0304-3835 ISO Abbreviation: Cancer Lett. Publication Date: 2007 Jan |
Date Detail:
|
Created Date: 2006-12-25 Completed Date: 2007-04-05 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7600053 Medline TA: Cancer Lett Country: Ireland |
Other Details:
|
Languages: eng Pagination: 156-62 Citation Subset: IM |
Affiliation:
|
Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Vienna, General Hospital of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, Austria. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adenosine Triphosphate
/
metabolism Apoptosis / drug effects Benzamidines / chemistry, pharmacology Cell Cycle / drug effects Cell Proliferation / drug effects Cell Survival / drug effects Cyclooxygenase Inhibitors / pharmacology* Cytidine Triphosphate / metabolism Dose-Response Relationship, Drug Enzyme Inhibitors / chemistry, pharmacology Gallic Acid / chemistry, pharmacology* Guanosine Triphosphate / metabolism HL-60 Cells Humans Leukemia, Promyelocytic, Acute / enzymology, metabolism, pathology Molecular Structure Prostaglandin-Endoperoxide Synthases / metabolism* Ribonucleotide Reductases / antagonists & inhibitors*, metabolism Thymine Nucleotides / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/3,4,5-trihydroxybenzamidoxime; 0/Benzamidines; 0/Cyclooxygenase Inhibitors; 0/Enzyme Inhibitors; 0/Thymine Nucleotides; 149-91-7/Gallic Acid; 365-08-2/thymidine 5'-triphosphate; 56-65-5/Adenosine Triphosphate; 65-47-4/Cytidine Triphosphate; 86-01-1/Guanosine Triphosphate; EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases; EC 1.17.4.-/Ribonucleotide Reductases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Classical pituitary tumour apoplexy: clinical features, management and outcomes in a series of 24 pa...
Next Document: Neoplastic transformation of human diploid fibroblasts after long-term serum starvation.