Document Detail

Gallic acid induces apoptosis of lung fibroblasts via a reactive oxygen species-dependent ataxia telangiectasia mutated-p53 activation pathway.
MedLine Citation:
PMID:  20151649     Owner:  NLM     Status:  MEDLINE    
Idiopathic pulmonary fibrosis (IPF) is a progressive chronic disorder characterized by the activation of fibroblasts and the overproduction of extracellular matrix. Fibroblast resistance to apoptosis leads to increased fibrosis. Targeting fibroblasts with apoptotic agents represents a major therapeutic intervention for debilitating IPF. Gallic acid (3,4,5-trihydroxybenzoic acid), a naturally occurring plant phenol, has been reported to induce apoptosis in tumor cell lines and renal fibroblasts. However, the effects of gallic acid on lung fibroblasts have not been investigated. The aim of the present study is to determine the effects of gallic acid on primary cultured mouse fibroblasts. Our results showed that gallic acid induces the apoptotic death of fibroblasts via both intrinsic and extrinsic apoptotic pathways by the elevation of PUMA, Fas, and FasL protein levels. Moreover, intracellular reactive oxygen species (ROS) generation and 8-hydroxy-2'-deoxyguanosine production were observed in gallic acid-stimulated fibroblasts. Mechanistic studies showed that gallic acid induces early phosphorylation of p53(Ser18) and histone 2AX(Ser139) (H2AX) via ataxia telangiectasia mutated (ATM) activation in response to ROS-provoked DNA damage. When mouse lung fibroblasts were treated with caffeine, an ATM kinase inhibitor, the levels of p53, phosphorylated p53(Ser18), and cell death induced by gallic acid were significantly attenuated. Additionally, pretreatment with antioxidants drastically inhibited the gallic acid-induced 8-hydroxy-2'-deoxyguanosine (8-OH-dG) formation and phosphorylation of p53(Ser18) and ATM(Ser1981), as well as apoptosis. Our results provide the first evidence of the activation of ROS-dependent ATM/p53 signaling as a critical mechanism of gallic acid-induced cell death in primary cultured mouse lung fibroblasts.
Cheng-Yen Chuang; Hsiang-Chun Liu; Li-Chen Wu; Chiu-Yuan Chen; Jinghua Tsai Chang; Shih-Lan Hsu
Related Documents :
17007439 - The use of hydroxyl-radical-generating systems for the treatment of olive mill wastewat...
17708639 - Metabolic characterization of brassica rapa leaves by nmr spectroscopy.
18588939 - Caffeic acid attenuates malathion induced metabolic disruption in rat liver, involvemen...
22244899 - Enhanced co-production of s-adenosylmethionine and glutathione by an atp-oriented amino...
11329689 - Algal growth inhibition effects and inducement modes by plant-producing phenols.
19719139 - Chemical composition and phenolic compound profile of morti??o (vaccinium floribundum k...
21565309 - Three aromatic amino acids in gastric juice as potential biomarkers for gastric maligna...
12935909 - Abietic acid activates peroxisome proliferator-activated receptor-gamma (ppargamma) in ...
2949389 - New chromogenic substrates for thrombin with increased specificity.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  58     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-03-03     Completed Date:  2010-07-19     Revised Date:  2011-11-02    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2943-51     Citation Subset:  IM    
Institute of Medical and Molecular Toxicology, Chung Shan Medical University, Taichung, Taiwan, ROC.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Apoptosis / drug effects*
Cell Cycle Proteins / metabolism*
Cells, Cultured
DNA Damage
DNA-Binding Proteins / metabolism*
Flow Cytometry
Gallic Acid / pharmacology*
Lung / cytology,  drug effects*,  metabolism
Protein-Serine-Threonine Kinases / metabolism*
Reactive Oxygen Species / metabolism*
Tumor Suppressor Protein p53 / metabolism*
Tumor Suppressor Proteins / metabolism*
Reg. No./Substance:
0/Cell Cycle Proteins; 0/DNA-Binding Proteins; 0/Reactive Oxygen Species; 0/Tumor Suppressor Protein p53; 0/Tumor Suppressor Proteins; 149-91-7/Gallic Acid; EC Kinases; EC telangiectasia mutated protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Influence of the Input System (Conventional versus Organic Farming) on Metabolite Profiles of Maize ...
Next Document:  Counting the Atoms in Supported, Monolayer-Protected Gold Clusters.