Document Detail


Galectin-3, an endogenous lectin, as a tool for monitoring cell differentiation in head and neck carcinomas with implications for lectin-glycan functionality.
MedLine Citation:
PMID:  12701754     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Galectin-3 is an endogenous lectin that reacts with glycan epitopes of membrane and extracellular glycoproteins, including integrins, fibronectin, laminin and tetraspanins. Its expression, and also the presentation of its glycoligands, is controlled in a differentiation-dependent manner in squamous epithelia. The aim of this study was to monitor the carbohydrate-dependent binding of labeled galectin-3 to primary head and neck squamous cell carcinomas (from the tonsil, base of the tongue and larynx) and lymph node metastases. MATERIAL AND METHODS: Double labeling (using antibodies against desmoplakin-1, Ki-67 and cytokeratins) at the single-cell level was employed to cytologically characterize cells reacting with galectin-3. RESULTS: Galectin-3 binds a non-proliferating pool of tumor cells. Colocalization of galectin-3 binding sites with desmosomal proteins may indicate a role for this endogenous lectin in the formation of intercellular contacts of the desmosomal type. Cytokeratin-10-positive tumor cells also presented galectin-3-reactive binding sites on the surface; however, cytokeratin-10-free cells were also recognized by this lectin. CONCLUSION: These findings intimate that galectin-3 may represent a new tool for monitoring the degree of cell differentiation in carcinomas originating from the transformation of squamous cell epithelia.
Authors:
Jan Betka; Jan Plzák; Karel Smetana; Hans-Joachim Gabius
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta oto-laryngologica     Volume:  123     ISSN:  0001-6489     ISO Abbreviation:  Acta Otolaryngol.     Publication Date:  2003 Jan 
Date Detail:
Created Date:  2003-04-18     Completed Date:  2003-07-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370354     Medline TA:  Acta Otolaryngol     Country:  Norway    
Other Details:
Languages:  eng     Pagination:  261-3     Citation Subset:  IM    
Affiliation:
Department of Otorhinolaryngology and Head and Neck Surgery, 1st Faculty of Medicine, Faculty Hospital Motol, Prague, Czech Republic. Jan.Betka@lfmotol.cuni.cz
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MeSH Terms
Descriptor/Qualifier:
Biopsy, Needle
Carcinoma, Squamous Cell / pathology*
Cell Differentiation / physiology*
Female
Galectin 3 / analysis,  metabolism*
Head and Neck Neoplasms / pathology*
Humans
Immunohistochemistry
Laryngeal Neoplasms / pathology
Lectins / analysis,  metabolism*
Ligands
Male
Neoplasm Staging
Sampling Studies
Sensitivity and Specificity
Tongue Neoplasms / pathology
Tonsillar Neoplasms / pathology
Tumor Markers, Biological / analysis*
Chemical
Reg. No./Substance:
0/Galectin 3; 0/Lectins; 0/Ligands; 0/Tumor Markers, Biological

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