Document Detail

Galanin's functional significance in the regulation of the neuroendocrine reproductive axis of the monkey.
MedLine Citation:
PMID:  10644895     Owner:  NLM     Status:  MEDLINE    
Galanin stimulates the neuroendocrine reproductive axis in the rat, but whether galanin acts similarly in primate species is unknown. To test the hypothesis that galanin acts within the hypothalamo-hypophyseal axis to stimulate luteinizing hormone (LH) and gonadotropin-releasing hormone (GnRH) secretion in the primate, galanin was administered either systemically or directly into the arcuate nucleus-median eminence of ovariectomized macaques (pigtailed or rhesus, respectively) that were maintained on estradiol. The mean plasma levels of LH were significantly elevated in pigtailed macaques after peripheral injection of galanin (2 mg) as compared with vehicle treatment. In rhesus monkeys, galanin (80 microM) administered by push-pull perfusion into the arcuate nucleus-median eminence did not significantly alter either GnRH or LH release. To determine whether in the monkey, as in the rat, subpopulations of medial forebrain GnRH neurons coexpress galanin mRNA, we used single- and double-label in situ hybridization and computerized imaging techniques. GnRH mRNA-containing cells were identified in both the medial and lateral forebrain of the female pigtailed macaque. No galanin mRNA expression was detectable in GnRH neurons located in either the medial preoptic area or mediobasal hypothalamus; however, within the substantia innominata a subset of GnRH mRNA-expressing neurons did coexpress galanin mRNA. Taken together, these results suggest that galanin induces LH release in primates, but galanin may not act directly on hypothalamic GnRH neurons. Presently, we have confirmed in another primate species the existence of GnRH gene expression in the lateral forebrain and discovered that a small subset of these neurons coexpress galanin. These particular cells may have a unique and as of yet undefined physiological function that is distinct from those GnRH neurons serving a hypophysiotropic function.
P D Finn; K Y Pau; H G Spies; M J Cunningham; D K Clifton; R A Steiner
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neuroendocrinology     Volume:  71     ISSN:  0028-3835     ISO Abbreviation:  Neuroendocrinology     Publication Date:  2000 Jan 
Date Detail:
Created Date:  2000-04-14     Completed Date:  2000-04-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0035665     Medline TA:  Neuroendocrinology     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  16-26     Citation Subset:  IM    
Copyright Information:
Copyright 2000 S. Karger AG, Basel.
Department of Physiology, University of Washington, Seattle, Wash., USA.
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MeSH Terms
Arcuate Nucleus / drug effects,  physiology
Drug Carriers
Estradiol / pharmacology
Galanin / genetics*,  metabolism,  pharmacology*
Gene Expression / physiology
Gonadotropin-Releasing Hormone / blood,  genetics,  secretion
In Situ Hybridization
Luteinizing Hormone / blood,  secretion
Macaca nemestrina
Median Eminence / drug effects,  physiology
Neurosecretory Systems / drug effects*,  physiology*
RNA, Messenger / analysis
Grant Support
Reg. No./Substance:
0/Dimethylpolysiloxanes; 0/Drug Carriers; 0/RNA, Messenger; 0/Silicones; 33515-09-2/Gonadotropin-Releasing Hormone; 50-28-2/Estradiol; 63148-62-9/baysilon; 88813-36-9/Galanin; 9002-67-9/Luteinizing Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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