Document Detail

Galanin and vasoactive intestinal polypeptide are colocalised with classical pituitary hormones and show plasticity of expression.
MedLine Citation:
PMID:  2482279     Owner:  NLM     Status:  MEDLINE    
The identity of galanin- and vasoactive intestinal polypeptide-(VIP) immunoreactive (IR) cells in the rat anterior pituitary was investigated using immunocytochemistry and, since levels of both peptides are stimulated by oestrogen, the effect of oestrogen treatment and gonadectomy on the expression of both peptides was examined. In normal male rats, few galanin-IR and very few VIP-IR cells were found. Colocalisation studies performed on 2-microns serial paraffin sections revealed that in these animals galanin IR was present in somatotrophs and thyrotrophs. In normal females in dioestrus many lactotrophs, in addition to somatotrophs and thyrotrophs, expressed galanin, but very few VIP-IR cells were seen. In cryostat sections of normal rat pituitaries, slightly more VIP-IR cells were present. Oestrogen treatment in females produced an increase in frequency of galanin-IR cells, the vast majority of which were lactotrophs, and more VIP-IR cells, identified as lactotrophs, also appeared. VIP was present in a subset of galanin-IR lactotrophs after oestrogen treatment. After ovariectomy female pituitaries resembled those of normal males, with few galanin positive cells none of which were lactotrophs, and hardly any VIP-IR cells. Thus these two peptides are present in specific endocrine cell types of rat anterior pituitary and display plasticity of expression in different cell types under the influence of oestrogen. Their roles in control of pituitary hormone secretion are supported by these findings, and it is possible that both peptides act in a paracrine fashion within the pituitary.
J H Steel; G Gon; D J O'Halloran; P M Jones; N Yanaihara; H Ishikawa; S R Bloom; J M Polak
Related Documents :
3345729 - Correlation of spatial differences in concentrations of prolactin and growth hormone ce...
7964269 - The effects of pyroglutamylglutamylprolineamide, a peptide related to thyrotrophin-rele...
11755719 - The effect of stimulators and blockers of adrenergic receptors on lh secretion and cycl...
6669039 - Continuous perifusion of dispersed anterior pituitary cells: technical aspects.
1705909 - The adenohypophysis of the australian lungfish, neoceratodus forsteri--an immunocytolog...
10698159 - Transforming growth factor-beta3 stimulates lactotrope cell growth by increasing basic ...
2581729 - Postnatal maturation of the islets of langerhans in sheep. light microscopic, immunohis...
19115959 - Method for the accurate preparation of cell-spiking standards.
24245939 - Flavonoids, flavonoid metabolites, and phenolic acids inhibit oxidative stress in the n...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Histochemistry     Volume:  93     ISSN:  0301-5564     ISO Abbreviation:  Histochemistry     Publication Date:  1989  
Date Detail:
Created Date:  1990-03-14     Completed Date:  1990-03-14     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0411300     Medline TA:  Histochemistry     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  183-9     Citation Subset:  IM    
Department of Histochemistry, Royal Postgraduate Medical School, Hammersmith Hospital, London, England.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Estradiol / pharmacology*
Frozen Sections
Peptides / analysis*,  metabolism
Pituitary Gland, Anterior / analysis*,  cytology
Pituitary Hormones / analysis*
Rats, Inbred Strains
Vasoactive Intestinal Peptide / analysis*,  metabolism
Reg. No./Substance:
0/Peptides; 0/Pituitary Hormones; 37221-79-7/Vasoactive Intestinal Peptide; 50-28-2/Estradiol; 8002-74-2/Paraffin; 88813-36-9/Galanin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Immunocytochemical detection of sulphonylated DNA in tissue sections. An alternative to Feulgen stai...
Next Document:  Lectin histochemical study of lipopigments with special regard to neuronal ceroid-lipofuscinosis. Re...