Document Detail


Galangin induces apoptosis of hepatocellular carcinoma cells via the mitochondrial pathway.
MedLine Citation:
PMID:  20632439     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To investigate the mechanism by which galangin, a polyphenolic compound derived from medicinal herbs, induces apoptosis of hepatocellular carcinoma (HCC) cells.
METHODS: The 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay was used to measure cell viability. Apoptosis was evaluated by in situ uptake of propidium iodide and Hoechst 33258 and was then detected by fluorescence microscopy. Protein expressions were detected by Western blotting. To confirm the apoptotic pathway mediated by galangin, cells were transfected by bcl-2 gene to overexpress Bcl-2 or siRNA to down-regulate Bcl-2 expression.
RESULTS: Galangin (46.25-370.0 micromol/L) exerted an anti-proliferative effect, induced apoptosis, and decreased mitochondrial membrane potential in a dose and time-dependent manner. Treatment with galangin induced apoptosis by translocating the pro-apoptotic protein Bax to the mitochondria, which released apoptosis-inducing factor and cytochrome c into the cytosol. Overexpression of Bcl-2 attenuated galangin-induced HepG2 cell apoptosis, while decreasing Bcl-2 expression enhanced galangin-induced cell apoptosis.
CONCLUSION: Our data suggests that galangin mediates apoptosis through a mitochondrial pathway, and may be a potential chemotherapeutic drug for the treatment of HCC.
Authors:
Hai-Tao Zhang; Hui Luo; Jun Wu; Liu-Bo Lan; Da-Hua Fan; Kai-Dan Zhu; Xiao-Yi Chen; Min Wen; Hui-Ming Liu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  16     ISSN:  2219-2840     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-07-15     Completed Date:  2010-10-18     Revised Date:  2014-05-20    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  3377-84     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / drug effects*
Carcinoma, Hepatocellular / drug therapy,  metabolism,  pathology*
Cell Line, Tumor / drug effects*,  metabolism,  pathology
Dose-Response Relationship, Drug
Flavonoids / pharmacology*,  therapeutic use
Humans
Liver Neoplasms / drug therapy,  metabolism,  pathology*
Membrane Potential, Mitochondrial / drug effects
Mitochondria / drug effects*,  metabolism
Mutagens / pharmacology*,  therapeutic use
Proto-Oncogene Proteins c-bcl-2 / genetics,  metabolism
Signal Transduction / drug effects,  physiology
Chemical
Reg. No./Substance:
0/Flavonoids; 0/Mutagens; 0/Proto-Oncogene Proteins c-bcl-2; 142FWE6ECS/galangin
Comments/Corrections

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