Document Detail

Galacturonic acid inhibits the growth of Saccharomyces cerevisiae on galactose, xylose, and arabinose.
MedLine Citation:
PMID:  22582063     Owner:  NLM     Status:  MEDLINE    
The efficient fermentation of mixed substrates is essential for the microbial conversion of second-generation feedstocks, including pectin-rich waste streams such as citrus peel and sugar beet pulp. Galacturonic acid is a major constituent of hydrolysates of these pectin-rich materials. The yeast Saccharomyces cerevisiae, the main producer of bioethanol, cannot use this sugar acid. The impact of galacturonic acid on alcoholic fermentation by S. cerevisiae was investigated with anaerobic batch cultures grown on mixtures of glucose and galactose at various galacturonic acid concentrations and on a mixture of glucose, xylose, and arabinose. In cultures grown at pH 5.0, which is well above the pK(a) value of galacturonic acid (3.51), the addition of 10 g · liter(-1) galacturonic acid did not affect galactose fermentation kinetics and growth. In cultures grown at pH 3.5, the addition of 10 g · liter(-1) galacturonic acid did not significantly affect glucose consumption. However, at this lower pH, galacturonic acid completely inhibited growth on galactose and reduced galactose consumption rates by 87%. Additionally, it was shown that galacturonic acid strongly inhibits the fermentation of xylose and arabinose by the engineered pentose-fermenting S. cerevisiae strain IMS0010. The data indicate that inhibition occurs when nondissociated galacturonic acid is present extracellularly and corroborate the hypothesis that a combination of a decreased substrate uptake rate due to competitive inhibition on Gal2p, an increased energy requirement to maintain cellular homeostasis, and/or an accumulation of galacturonic acid 1-phosphate contributes to the inhibition. The role of galacturonic acid as an inhibitor of sugar fermentation should be considered in the design of yeast fermentation processes based on pectin-rich feedstocks.
Eline H Huisjes; Erik de Hulster; Jan C van Dam; Jack T Pronk; Antonius J A van Maris
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-05-11
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  78     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-07-19     Completed Date:  2012-12-10     Revised Date:  2013-06-25    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5052-9     Citation Subset:  IM    
Department of Biotechnology, Delft University of Technology and Kluyver Centre for Genomics of Industrial Fermentation, Delft, The Netherlands.
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MeSH Terms
Animal Feed
Arabinose / metabolism*
Culture Media / metabolism*
Dose-Response Relationship, Drug
Fermentation / drug effects
Flow Cytometry
Galactose / metabolism*
Hexuronic Acids / pharmacology*
Hydrogen-Ion Concentration
Saccharomyces cerevisiae / drug effects*,  growth & development*,  metabolism
Xylose / metabolism*
Reg. No./Substance:
0/Culture Media; 0/Hexuronic Acids; 0/Xylose; 147-81-9/Arabinose; 26566-61-0/Galactose; 4JK6RN80GF/galacturonic acid

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